Myanmar traditional medicine formulations and their antioxidant, antiglycation and alpha-glucosidase inhibitory activities: Potentials for antidiabetes complications

Abstract

Myanmar Traditional Medicine (MTM) has been used since ancient times to treat life-threatening diseases like diabetes. In this study, various in vitro assays were used to prove that four MTM formulations were effective at treating diabetes. Antioxidant activities were determined using in vitro DPPH, nitric oxide (NO), and superoxide (SO) radical scavenging assays. The Folin-Ciocalteu method was used to quantify the total phenolic content, while the BSA-fluorescent antiglycation and α-glucosidase inhibitory assays were utilized to determine the antidiabetic activity of MTMs. Among the tested samples, MTM3 showed the best activities for almost all the biological assays tested in this experiment with the % inhibition of 82.89 ± 1.64 for NO and 65.02 ± 2.82 for SO radical scavenging activity, 92.12 ± 1.18 for α-glucosidase inhibitory activity and IC 50 of 180.29 ± 1.6 µg/ml for the antiglycation activity. It also possessed the highest total phenolic content of 149.41 ± 3.7 mg GAE/g of extract/l among the tested samples. Therefore, the findings suggested that MTM could help diabetic patients improve their quality of life through antioxidant activity against several free radicals and their antiglycation and α-glucosidase inhibitory characteristics.

Keywords

References

1. Abu-Zaiton, A. S. (2010). Anti-diabetic activity of Ferula assafoetida extract in normal and alloxan-induced diabetic rats. Pakistan Journal of Biological Sciences, 13(2), 97-100.
2. Den Hartogh, D. J., Gabriel, A., & Tsiani, E. (2019). Antidiabetic properties of curcumin II: evidence from in vivo studies. Nutrients, 12(1), 58.
3. Fazel Nabavi, S., Thiagarajan, R., Rastrelli, L., Daglia, M., Sobarzo-Sanchez, E., Alinezhad, H., & Mohammad Nabavi, S. (2015). Curcumin: a natural product for diabetes and its complications. Current Topics in Medicinal Chemistry, 15(23), 2445-2455.
4. Geberemeskel, G. A., Debebe, Y. G., & Nguse, N. A. (2019). Antidiabetic effect of fenugreek seed powder solution (Trigonella foenum-graecum L.) on hyperlipidemia in diabetic patients. Journal of Diabetes Research, 2019, 8507453.
5. Hertog, M. G., Hollman, P. C., & Van de Putte, B. (1993). Content of potentially anticarcinogenic flavonoids of tea infusions, wines, and fruit juices. Journal of Agricultural and Food Chemistry, 41(8), 1242-1246.
6. Joladarashi, D., Chilkunda, N. D., & Salimath, P. V. (2014). Glucose uptake-stimulatory activity of Tinospora cordifolia stem extracts in Ehrlich ascites tumor cell model system. Journal of Food Science and Technology, 51, 178-182.
7. Krishnakumar, I., Issac, A., Johannah, N., Ninan, E., Maliakel, B., & Kuttan, R. (2014). Effects of the polyphenol content on the anti-diabetic activity of Cinnamomum zeylanicum extracts. Food & Function, 5(9), 2208-2220.
8. Latifi, E., Mohammadpour, A. A., Fathi, B., & Nourani, H. (2019). Antidiabetic and antihyperlipidemic effects of ethanolic Ferula assa-foetida oleo-gum-resin extract in streptozotocin-induced diabetic wistar rats. Biomedicine & Pharmacotherapy, 110, 197-202.
9. Lee, S. K., Mbwambo, Z. H., Chung, H. S., Luyengi, L., Gamez, E. J., Mehta, R. G., Kinghorn, A. D., & Pezzuto, J. M. (1998). Evaluation of the antioxidant potential of natural products. Combinatorial Chemistry and High Throughput Screening, 1(1), 35-46.
10. Mamatha, D. (2017). Formulation and evaluation of poly herbal anti-diabetic tablet dosage form. International Journal of Ayurvedic & Herbal Medicine, 7(6), 2956-2962.
11. Matough, F. A., Budin, S. B., Hamid, Z. A., Alwahaibi, N., & Mohamed, J. (2012). The role of oxidative stress and antioxidants in diabetic complications. Sultan Qaboos University Medical Journal, 12(1), 5-18.
12. Ministry of Health. (2006). Health in Myanmar (K. Myint Ed.).
13. Moe, T. S., Win, H. H., Hlaing, T. T., Lwin, W. W., Htet, Z. M., & Mya, K. M. (2018). Evaluation of in vitro antioxidant, antiglycation and antimicrobial potential of indigenous Myanmar medicinal plants. Journal of Integrative Medicine, 16(5), 358-366.
14. Moradi, B., Abbaszadeh, S., Shahsavari, S., Alizadeh, M., & Beyranvand, F. (2018). The most useful medicinal herbs to treat diabetes. Biomedical Research and Therapy, 5(8), 2538-2551.
15. Noor, H., & Ashcroft, S. J. (1998). Pharmacological characterisation of the antihyperglycaemic properties of Tinospora crispa extract. Journal of Ethnopharmacology, 62(1), 7-13.
16. Noor, H., Hammonds, P., Sutton, R., & Ashcroft, S. (1989). The hypoglycaemic and insulinotropic activity of Tinospora crispa: studies with human and rat islets and HIT-T15 B cells. Diabetologia, 32, 354-359.
17. Nugroho, A. E., Andrie, M., Warditiani, N. K., Siswanto, E., Pramono, S., & Lukitaningsih, E. (2012). Antidiabetic and antihiperlipidemic effect of Andrographis paniculata (Burm. f.) Nees and andrographolide in high-fructose-fat-fed rats. Indian Journal of Pharmacology, 44(3), 377-381.
18. Patel Rajesh, M., & Patel Natvar, J. (2011). In vitro antioxidant activity of coumarin compounds by DPPH, super oxide and nitric oxide free radical scavenging methods. Journal of Advanced Pharmacy Education & Research, 1(1), 52-68.
19. Perera, P., Ekanayaka, S., & Ranaweera, K. (2013). In vitro antiglycation activity of some medicinal plants used in diabetes mellitus. Medicinal Aromatic Plants, 2(6), 143-145.
20. Rafe, M. R. (2017). A review of five traditionally used anti-diabetic plants of Bangladesh and their pharmacological activities. Asian Pacific Journal of Tropical Medicine, 10(10), 933-939.
21. Sharafeldin, K., & Rizvi, M. R. (2015). Effect of traditional plant medicines (Cinnamomum zeylanicum and Syzygium cumini) on oxidative stress and insulin resistance in streptozotocin-induced diabetic rats. The Journal of Basic & Applied Zoology, 72, 126-134.
22. Vhora, N., Naskar, U., Hiray, A., Kate, A. S., & Jain, A. (2020). Recent advances in in-vitro assays for type 2 diabetes mellitus: An overview. Review of Diabetic Studies, 16(1), 13-23.
23. Wai, K. (2015). Taking stock of traditional medicine. Myanmar Times. Retrieved from https://www.mmtimes.com/
24. Waterhouse, A. L. (2002). Determination of total phenolics. Current Protocols in Food Analytical Chemistry, 6(1), I1. 1.1-I1. 1.8.
25. Yarizade, A., Kumleh, H. H., & Niazi, A. (2017). In vitro antidiabetic effects of Ferula assa-foetida extracts through dipeptidyl peptidase iv and α-glucosidase inhibitory activity. In Vitro, 10(5), 357-360.
26. Yongchaiyudha, S., Rungpitarangsi, V., Bunyapraphatsara, N., & Chokechaijaroenporn, O. (1996). Antidiabetic activity of Aloe vera L. juice. I. Clinical trial in new cases of diabetes mellitus. Phytomedicine, 3(3), 241-243.

License

Copyright (c) 2024 The Su Moe, Htet Htet Win, Zar Kyi Win, Nwe Nwe Htay, Mya Thida