Therapeutic effect of the solvent fraction of hexane leaf extract of Tapinanthus bangwensis (Engl. & K. Krause) (Loranthaceae) in alloxan-induced pathology in diabetic rats

Godwin Okwudiri Ihegboro; Chimaobi James Ononamadu; Mujiburrahman Fadilu; Peter Prince Oghenekome; Bello Jacob; Sunday Edwin

doi:10.62313/ijpbp.2024.196

Authors

Godwin Okwudiri Ihegboro Nigeria Police Academy, Faculty of Science, Department of Biochemistry and Forensic Science, Wudil, Kano, Nigeria https://orcid.org/0000-0002-4032-3475
Chimaobi James Ononamadu Nigeria Police Academy, Faculty of Science, Department of Biochemistry and Forensic Science, Wudil, Kano, Nigeria https://orcid.org/0000-0001-9561-6075
Mujiburrahman Fadilu Nigeria Police Academy, Faculty of Science, Department of Biochemistry and Forensic Science, Wudil, Kano, Nigeria https://orcid.org/0000-0001-6356-6085
Peter Prince Oghenekome Nigeria Police Academy, Faculty of Science, Department of Biochemistry and Forensic Science, Wudil, Kano, Nigeria https://orcid.org/0009-0004-3939-1739
Bello Jacob Nigeria Police Academy, Faculty of Science, Department of Biochemistry and Forensic Science, Wudil, Kano, Nigeria https://orcid.org/0009-0009-4373-9917
Sunday Edwin Nigeria Police Academy, Faculty of Science, Department of Biochemistry and Forensic Science, Wudil, Kano, Nigeria https://orcid.org/0009-0001-7604-4937

DOI:

https://doi.org/10.62313/ijpbp.2024.196

Keywords:

Oxidative stress, Tapinanthus bangwensis, Diabetic rats, Haematopoietic markers, Silymarin tablets

Abstract

Plant-based products are gradually replacing pharmaceuticals in treating ailments, including diabetes, due to their safety, cost-effectiveness, potency, and availability. Therefore, the current study looked into the therapeutic effect of the solvent fraction of hexane leaf extract of Tapinanthus bangwensis (HEXETACF) (Loranthaceae) in alloxan-induced pathology in diabetic rats. The biochemical parameters were estimated using analytical grade kits via spectrophotometric method. The laboratory rats were distributed into group W (five rats on feed and water), group X (seven rats + 150 mg/kg alloxan solution only), group Y (seven rats + 150 mg/kg alloxan solution + 200 mg/kg BW silymarin for 21 days), and group Z (seven rats + 150 mg/kg alloxan solution + 250 mg/kg BW HEXETACF for 21 days). The results showed that HEXETACF and silymarin (SILY) reduced blood glucose concentration by 33.77% and 34.80%, respectively, after the 21^st day of treatment (p < 0.05). Additionally, alkaline phosphatase (ALP) and alanine aminotransferase (ALT) activity in SILY and HEXETACF were significantly decreased compared to the diabetic group (p < 0.05), but no significant decrease in aspartate aminotransferase (AST) activity was observed between the test samples and the diabetic group. Furthermore, the test samples lowered malondialdehyde (MDA) levels, by improving glutathione, superoxide dismutase (SOD), and catalase (CAT) activity. The HEXETACF and SILY significantly decreased triglyceride levels (TG) compared to the diabetic group at p < 0.05. They also reduced low-density lipoprotein (LDL) and cholesterol levels and increased the high-density lipoprotein levels compared to the diabetic group. Additionally, no significant decrease in serum electrolytes (Na⁺, K⁺, and Cl^-), urea, and creatinine (including albumin and total protein) values was observed in HEXETACF and SILY, while hematological indices increased compared to the diabetic group. Histology results revealed that the test samples had normalized glomeruli, β-islet cells, and hepatocytes. However, a trace of mild congestion was noticed in the STDG. But edemic blood congestion was observed in the diabetic group. In conclusion, the current result demonstrated that HEXETACF may be a promising antidiabetic agent that could replace mSILY.

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