Pergularia daemia (Apocynaceae) mitigates rifampicin-induced hepato-renal injury: potentials in the management of liver and kidney diseases

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Pergularia daemia, Liver, Kidney, Biomarkers, Toxicity, Rifampicin


Medicinal potentials of Pergularia daemia leaves in managing hepato-renal toxicity induced by rifampicin were investigated. Twenty-five (25) Wistar rats were randomly placed into five groups containing five animals each. All the animals, except group I, were orally exposed to 250 g/kg bwt rifampicin and administered different treatments. Specific liver and kidney biomarkers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were determined. In addition, malondialdehyde (MDA), lipid profile, superoxide dismutase (SOD), catalase (CAT), as well as reduced glutathione (GSH) were determined in the serum, liver, and kidney homogenates of experimental animals. Results indicate that exposure to rifampicin caused significant depletion in SOD and CAT relative to the control animals. Lipid profile was deranged, while ALT, AST, ALP, urea, uric acid, bilirubin, creatine kinase, and MDA level were elevated by rifampicin exposure. All deranged biochemical indices, as well as distorted histoarchitecture, were restored dose-dependently after treatment with P. daemia. In conclusion, P. daemia ameliorated rifampicin toxicity on the liver and kidney as indicated in the restoration of all deranged biochemical and histopathological indices measured. Hence, it is a potential therapeutic agent that can be harnessed as the panacea to the menace of liver and kidney diseases.


Abirami, A., Nagarani, G., Siddhuraju, P., 2014. In vitro antioxidant, anti-diabetic, cholinesterase and tyrosinase inhibitory potential of fresh juice from Citrus hystrix and C. maxima fruits. Food Science and Human Wellness, 3(1), 16-25.

Antonyuk, S.V., Strange, R.W., Marklund, S.L., Hasnain, S.S., 2009. The structure of human extracellular copper–zinc superoxide dismutase at 1.7 Å resolution: insights into heparin and collagen binding. Journal of Molecular Biology, 388(2), 310-326.

Balakrishnan, S., Khurana, B.S., Singh, A., Kaliappan, I., Dubey, G.P., 2012. Hepatoprotective effect of hydroalcoholic extract of Cissampelos pareira against rifampicin and isoniazid induced hepatotoxicity. Continental Journal of Food Science and Technology, 6(1), 30-35.

Basheer, A.S., Siddiqui, A., Paudel, Y.N., Hassan, M.Q., Imran, M., Najmi, A.K., Akhtar, M., 2017. Hepatoprotective and antioxidant effects of fish oil on isoniazid-rifampin induced hepatotoxicity in rats. PharmaNutrition, 5(1), 29-33.

Beutler, E., 1963. Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine, 61, 882-888.

Bhusari, S., Bhokare, S.G., Nikam, K.D., Chaudhary, A.N., Wakte, P.S., 2018. Pharmacognostic and Phytochemical investigation of stems of Pergularia daemia. Asian Journal of Pharmacy and Pharmacology, 4(4), 500-504.

Biour, J.M., Tymoczko, J.L., Stryer, L., 2004. Biochemistry. W.H. Freeman. pp. 656–660. ISBN 978-0-7167-8724-2.

Brehe, J.E., Burch, H.B., 1976. Enzymatic assay for glutathione. Analytical Biochemistry, 74(1), 189-197.

Byrne, J.A., Strautnieks, S.S., Mieli–Vergani, G., Higgins, C.F., Linton, K.J., Thompson, R.J., 2002. The human bile salt export pump: characterization of substrate specificity and identification of inhibitors. Gastroenterology, 123(5), 1649-1658.

Capelle, P., Dhumeaux, D., Mora, M., Feldmann, G., Berthelot, P., 1972. Effect of rifampicin on liver function in man. Gut, 13(5), 366-371.

Chandak, R.R., 2010. Preliminary Phytochemical Investigation of Pergularia daemia linnInt. Journal of Pharmaceutical Studies & Research, 1(1), 11-16.

Chandak, R.R., Dighe, N.S., 2019. A Review on Phytochemical & Pharmacological Profile of Pergularia daemia linn. Journal of Drug Delivery and Therapeutics, 9(4-s), 809-814.

Dosumu, O.O., Ajetumobi, O.O., Omole, O.A., Onocha, P.A., 2019. Phytochemical composition and antioxidant and antimicrobial activities of Pergularia daemia. Journal of Medicinal Plants for Economic Development, 3(1), 1-8.

Eminizade, N., Krance, S.M., Notenboom, S., Shi, S., Tieu, K., Hammond, C.L., 2008. Glutathione dysregulation and the etiology and progression of human diseases. Biological Chemistry, 390(3), 191-214.

Englehardt, A., 1970. Measurement of alkaline phosphatase. Aerztl Labor, 16(42), 1.

Espinosa-Diez, C., Miguel, V., Mennerich, D., Kietzmann, T., Sánchez-Pérez, P., Cadenas, S., Lamas, S., 2015. Antioxidant responses and cellular adjustments to oxidative stress. Redox Biology, 6, 183-197.

Friedewald, W.T., Levy, R.I., Fredrickson, D.S., 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry, 18, 499–502.

Grosset, J., Leventis, S., 1983. Adverse effects of rifampin. Reviews of Infectious Diseases, 5(Supplement_3), S440-S446.

Grove, T.H., 1979. Effect of reagent pH on determination of high-density lipoprotein cholesterol by precipitation with sodium phosphotungstate-magnesium. Clinical Chemistry, 25(4), 560-564.

Heit, C., Marshall, S., Singh, S., Yu, X., Charkoftaki, G., Zhao, H., Vasiliou, V., 2017. Catalase deletion promotes prediabetic phenotype in mice. Free Radical Biology and Medicine, 103, 48-56.

Jaswal, A., Sinha, N., Bhadauria, M., Shrivastava, S., Shukla, S., 2013. Therapeutic potential of thymoquinone against anti-tuberculosis drugs induced liver damage. Environmental Toxicology and Pharmacology, 36(3), 779-786.

Jaydeokar, A.V., Bandawane, D.D., Bibave, K.H., Patil, T.V., 2014. Hepatoprotective potential of Cassia auriculata roots on ethanol and antitubercular drug-induced hepatotoxicity in experimental models. Pharmaceutical Biology, 52(3), 344-355.

Jelkmann, W., 2001. The role of the liver in the production of thrombopoietin compared with erythropoietin. European Journal of Gastroenterology & Hepatology, 13(7), 791-801.

Kim, J.H., Nam, W.S., Kim, S.J., Kwon, O.K., Seung, E.J., Jo, J.J., Lee, S., 2017. Mechanism investigation of rifampicin-induced liver injury using comparative toxicoproteomics in mice. International Journal of Molecular Sciences, 18(7), 1417.

Kohli, H.S., Bhaskaran, M.C., Muthukumar, T., Thennarasu, K., Sud, K., Jha, V., Sakhuja, V., 2000. Treatment-related acute renal failure in the elderly: a hospital-based prospective study. Nephrology Dialysis Transplantation, 15(2), 212-217.

Kosanam, S., Boyina, R., 2015. Drug-induced liver injury: A review. International Journal of Pharmacological Research, 5(2), 24-30.

Krishnaiah, D., Sarbatly, R., Nithyanandam, R., 2011. A review of the antioxidant potential of medicinal plant species. Food and Bioproducts Processing, 89(3), 217-233.

Larrey, D., 2000. Drug-induced liver diseases. Journal of Hepatology, 32, 77-88.

Lee, C.L., Sherman, P.M., 2000. Pediatric Gastrointestinal Disease. Connecticut: PMPH-USA. p. 751. ISBN 978-1-55009-364-3.

Maheshwari, M., Vijayarengan, P., 2021. Phytochemical Evaluation, FT-IR and GC-MS Analysis of Leaf Extracts of Pergularia daemia. Nature Environment and Pollution Technology, 20(1), 259-265.

Misra, H.P., Fridovich, I., 1972. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry, 247(10), 3170-3175.

Mohammed, S., Kasera, P.K., Shukla, J.K., 2004. Unexploited plants of potential medicinal value from the Indian Thar desert. Natural Product Radiance, 3, 69-74.

Muller, F.L., Song, W., Liu, Y., Chaudhuri, A., Pieke-Dahl, S., Strong, R., Van Remmen, H., 2006. Absence of CuZn superoxide dismutase leads to elevated oxidative stress and acceleration of age-dependent skeletal muscle atrophy. Free Radical Biology and Medicine, 40(11), 1993-2004.

Naik, S.R., Panda, V.S., 2008. Hepatoprotective effect of Ginkgoselect Phytosome® in rifampicin induced liver injurym in rats: Evidence of antioxidant activity. Fitoterapia, 79(6), 439-445.

Nithyatharani, R., Kavitha, U., 2018. Phytochemical Studies on the Leaves of Pergularia daemia Collected from Villupuram District, Tamil Nadu, India. IOSR Journal of Pharmacy, 8(1), 9-12.

Panich, U., Onkoksoong, T., Limsaengurai, S., Akarasereenont, P., Wongkajornsilp, A., 2012. UVA-induced melanogenesis and modulation of glutathione redox system in different melanoma cell lines: the protective effect of gallic acid. Journal of Photochemistry and Photobiology B: Biology, 108, 16-22.

Rana, S.V., Pal, R., Vaiphie, K., Singh, K., 2006. Effect of different oral doses of isoniazid-rifampicin in rats. Molecular and Cellular Biochemistry, 289(1), 39-47.

Reitman, S., Frankel, S., 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology, 28(1), 56-63.

Renugadevi, J., Prabu, S.M., 2009. Naringenin protects against cadmium-induced oxidative renal dysfunction in rats. Toxicology, 256(1-2), 128-134.

Renugadevi, J., Prabu, S.M., 2010. Cadmium-induced hepatotoxicity in rats and the protective effect of naringenin. Experimental and Toxicologic Pathology, 62(2), 171-181.

Santhosh, S., Sini, T.K., Anandan, R., Mathew, P.T., 2006. Effect of chitosan supplementation on antitubercular drugs-induced hepatotoxicity in rats. Toxicology, 219(1-3), 53-59.

Saukkonen, J.J., Cohn, D.L., Jasmer, R.M., Schenker, S., Jereb, J.A., Nolan, C.M., 2006. On the behalf of ATS (American Thoracic Society). Hepatotoxicity of Antituberculosis Therapy. American Journal of Respiratory and Critical Care Medicine, 174(8), 935-952.

Sedlak, T.W., Snyder, S.H., 2004. Bilirubin benefits: cellular protection by a biliverdin reductase antioxidant cycle. Pediatrics, 113(6), 1776-1782.

Sentman, M.L., Granström, M., Jakobson, H., Reaume, A., Basu, S., Marklund, S.L., 2006. Phenotypes of mice lacking extracellular superoxide dismutase and copper-and zinc-containing superoxide dismutase. Journal of Biological Chemistry, 281(11), 6904-6909.

Sharma, R., Sharma, V.L., 2015. Review: treatment of toxicity caused by anti-tubercular drugs by use of different herbs. International Journal of Pharma Sciences and Research, 6(10), 1288-1294.

Shukla, S., Sinha, N., Jaswal, A., 2014. Anti Oxidative, Anti Peroxidative and Hepatoprotective Potential of Phyllanthus amarus Against Anti Tb Drugs. In Pharmacology and Nutritional Intervention in the Treatment of Disease. IntechOpen, 2014, 283-294.

Sidhu, D., Naugler, C., 2012. Fasting time and lipid levels in a community-based population: a cross-sectional study. Archives of Internal Medicine, 172(22), 1707-1710.

Sinha, A.K., 1972. Colorimetric assay of catalase. Analytical Biochemistry, 47(2), 389-394.

Tasduq, S.A., Kaiser, P., Sharma, S.C., Johri, R.K., 2007. Potentiation of isoniazid‐induced liver toxicity by rifampicin in a combinational therapy of antitubercular drugs (rifampicin, isoniazid and pyrazinamide) in Wistar rats: A toxicity profile study. Hepatology Research, 37(10), 845-853.

Tietz, N.W., 1995. Clinical Guide to Laboratory Tests, 3rd Edition, W.B. Saunders, Philadelphia.

Trinder, P., 1969. A simple Turbidimetric method for the determination of serum cholesterol. Annals of Clinical Biochemistry, 6(5), 165-166.

Ueno, Y., Kizaki, M., Nakagiri, R., Kamiya, T., Sumi, H., Osawa, T., 2002. Dietary glutathione protects rats from diabetic nephropathy and neuropathy. The Journal of Nutrition, 132(5), 897-900.

Vaithiyanathan, V., Mirunalini, S., 2015. Quantitative variation of bioactive phyto compounds in ethyl acetate and methanol extracts of Pergularia daemia (Forsk.) Chiov. Journal of Biomedical Research, 29(2), 169-172.

Vaithiyanathan, V., Mirunalini, S., 2016. Assessment of anticancer activity: A comparison of dose–response effect of ethyl acetate and methanolic extracts of Pergularia daemia (Forsk). Oral Science International, 13(1), 24-31.

Vanderlinde, R.E., 1981. Urinary enzyme measurements in the diagnosis of renal disorders. Annals of Clinical & Laboratory Science, 11(3), 189-201.

Verma, P., Paswan, S., Singh, S.P., Shrivastva, S., Rao, C.V., 2015. Assessment of hepatoprotective potential of Solanum xanthocarpum (whole plant) Linn. against isoniazid & rifampicin induced hepatic toxicity in Wistar rats. Indian Journal of Research in Pharmacy and Biotechnology, 3, 373-379.

Weichselbaum, C.T., 1946. An accurate and rapid method for the determination of proteins in small amounts of blood serum and plasma. American Journal of Clinical Pathology, 16(3_ts), 40-49.

Yue-Ming, W., Sergio, C.C., Christopher, T.B., Taoshen, C., 2014. Pregnane X receptor and drug-induced liver injury expert Opin. Journal of Drug Metabolism & Toxicology, 10(11), 1521-1532.




How to Cite

Ogunmoyole, T., Fatile, O. G., Johnson, O. D., & Yusuff, A. A. (2022). Pergularia daemia (Apocynaceae) mitigates rifampicin-induced hepato-renal injury: potentials in the management of liver and kidney diseases. International Journal of Plant Based Pharmaceuticals, 2(2), 196–204.



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