The inclusion of garlic and turmeric powder in high-fructose diets protects against the development of metabolic syndrome in Wistar rats

Abstract

The worldwide prevalence of the metabolic syndrome is largely attributable to excessive consumption of high-energy food sweeteners and lifestyle practices that encourage physical inactivity. This study was designed to evaluate the potential benefits of garlic and/or turmeric in down-regulating the risk factors associated with metabolic syndrome. Twenty-four male Wistar rats were divided into 8 groups of 3 rats per group. Group 1 received standard rat chow, Group 2 received a high-fructose diet only, while Group 3 received a 2% turmeric-supplemented high-fructose diet. Groups 4 and 5 were fed a standard diet supplemented with 2% each of garlic and turmeric:garlic (50% w/w), respectively. Groups 6, 7, and 8 were respectively fed a high-fructose diet supplemented with 2% garlic, a standard diet supplemented with 2% turmeric, and a high-fructose diet supplemented with 2% turmeric:garlic (50% w/w). Feed intake and changes in body weight were monitored weekly and after 56 days, the rats were sacrificed. Activities of serum antioxidant enzymes, lipid profile, and atherogenic indices were determined. Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) was computed from the measured blood glucose and insulin levels. The positive control (Group 2) gained weight significantly ( p < 0.05) when compared with groups that received diet supplementation. Total cholesterol, triglycerides, low-density lipoprotein, and atherogenic indices of diet-supplemented groups were significantly low ( p < 0.05) when compared with the positive control. Interestingly, while no differences ( p > 0.05) were observed in the catalase and glutathione peroxidase enzyme activities in the high-fat diet group supplemented with 2% mixed turmeric:garlic when compared with the normal control, activities of these enzymes in the garlic and/or turmeric supplemented high-fructose diet groups were significantly elevated ( p < 0.05) when compared with the positive control. The HOMA-IR and atherogenic indices results revealed the inclusion of turmeric and garlic in a high-fat diet had anti-dyslipidemic effects, decreased oxidative stress, and reduced coronary risk factors. Our findings strongly suggest supplementation of high-calorie diets with garlic and/or turmeric powder has potential long-term health benefits in individuals exposed to the risks of developing metabolic syndrome.

Keywords

References

1. Afrin, R., Arumugam, S., Rahman, A., Wahed, M. I. I., Karuppagounder, V., Harima, M., Suzuki, H., Miyashita, S., Suzuki, K., et al. (2017). Curcumin ameliorates liver damage and progression of NASH in NASH-HCC mouse model possibly by modulating HMGB1-NF-κB translocation. International Immunopharmacology, 44, 174-182.
2. Alberti, K. G., Eckel, R. H., Grundy, S. M., Zimmet, P. Z., Cleeman, J. I., Donato, K. A., Fruchart, J. C., James, W. P. T., Loria, C. M., et al. (2009). Harmonizing the metabolic syndrome: a joint interim statement of the international diabetes federation task force on epidemiology and prevention; national heart, lung, and blood institute; American heart association; world heart federation; international atherosclerosis society; and international association for the study of obesity. Circulation, 120(16), 1640-1645.
3. Anand, P., Kunnumakkara, A. B., Newman, R. A., & Aggarwal, B. B. (2007). Bioavailability of curcumin: problems and promises. Molecular Pharmaceutics, 4(6), 807-818.
4. Arslan, S., & Şanlıer, N. (2016). Relationship between daily dietary fructose intake, body composition and biochemical parameters patients with type 2 diabetes. Journal of Human Sciences, 13(2), 2642-2655.
5. Coban, D., Milenkovic, D., Chanet, A., Khallou‐Laschet, J., Sabbe, L., Palagani, A., Vanden Berghe, W., Mazur, A., & Morand, C. (2012). Dietary curcumin inhibits atherosclerosis by affecting the expression of genes involved in leukocyte adhesion and transendothelial migration. Molecular Nutrition & Food Research, 56(8), 1270-1281.
6. National Research Council (2011). Guide for the care and use of laboratory animals, National Academies Press. Washington, DC.
7. do Vale Moreira, N. C., Hussain, A., Bhowmik, B., Mdala, I., Siddiquee, T., Fernandes, V. O., Júnior, R. M. M., & Meyer, H. E. (2020). Prevalence of metabolic syndrome by different definitions, and its association with type 2 diabetes, pre-diabetes, and cardiovascular disease risk in Brazil. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 14(5), 1217-1224.
8. Engin, A. (2017). The definition and prevalence of obesity and metabolic syndrome. Obesity and Lipotoxicity. Advances in Experimental Medicine and Biology, (Vol. 960): Springer, Cham.
9. Friedewald, W. T., Levy, R. I., & Fredrickson, D. S. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry, 18(6), 499-502.
10. Gierach, M., Gierach, J., Ewertowska, M., Arndt, A., & Junik, R. (2014). Correlation between body mass index and waist circumference in patients with metabolic syndrome. International Scholarly Research Notices, 2014, 514589.
11. Ginsberg, H. N., Zhang, Y. L., & Hernandez-Ono, A. (2006). Metabolic syndrome: focus on dyslipidemia. Obesity, 14(2S), 41S-49S.
12. Gupta, N., & Porter, T. D. (2001). Garlic and garlic-derived compounds inhibit human squalene monooxygenase. The Journal of Nutrition, 131(6), 1662-1667.
13. Hannou, S. A., Haslam, D. E., McKeown, N. M., & Herman, M. A. (2018). Fructose metabolism and metabolic disease. The Journal of Clinical Investigation, 128(2), 545-555.
14. Herman, M. A., & Samuel, V. T. (2016). The sweet path to metabolic demise: fructose and lipid synthesis. Trends in Endocrinology & Metabolism, 27(10), 719-730.
15. Huang, C. J., McAllister, M. J., Slusher, A. L., Webb, H. E., Mock, J. T., & Acevedo, E. O. (2015). Obesity-related oxidative stress: the impact of physical activity and diet manipulation. Sports Medicine-Open, 1(1), 32.
16. Idoko, A., Abdullahi, A., Maibulangu, B., Nura, L., Imam, N., Bonomi, Z., Muhammed, F., & Umar, S. (2022). Allium sativum and Curcuma longa Powder Protect Against Hepatotoxic and Nephrotoxic Effects of High-fructose Diet. FUOYE Journal of Pure and Applied Sciences (FJPAS), 7(1), 59-78.
17. Imam, N., Idoko, A., Osibemhe, M., Lawal, N., & Zaharaddeen, A. (2022). Obesity and Insulin Resistance Components of Metabolic Syndrome Induced by High-fructose Diet in Wistar Rats could be attenuated by Spices-Supplemented Diets. Journal of Applied Sciences and Environmental Management, 26(5), 893-901.
18. Johnston, T., Korolenko, T., Pirro, M., & Sahebkar, A. (2017). Preventing cardiovascular heart disease: Promising nutraceutical and non-nutraceutical treatments for cholesterol management. Pharmacological Research, 120, 219-225.
19. Kang, C., & Kim, E. (2010). Synergistic effect of curcumin and insulin on muscle cell glucose metabolism. Food and Chemical Toxicology, 48(8-9), 2366-2373.
20. Kazemi, T., Hajihosseini, M., Moossavi, M., Hemmati, M., & Ziaee, M. (2018). Cardiovascular risk factors and atherogenic indices in an Iranian population: Birjand East of Iran. Clinical Medicine Insights: Cardiology, 12, 1179546818759286.
21. Kim, M., & Kim, H. (2011). Effect of garlic on high fat induced obesity. Acta Biologica Hungarica, 62(3), 244-254.
22. Lai, Y. S., Chen, W. C., Ho, C. T., Lu, K. H., Lin, S. H., Tseng, H. C., Lin, S. Y., & Sheen, L. Y. (2014). Garlic essential oil protects against obesity-triggered nonalcoholic fatty liver disease through modulation of lipid metabolism and oxidative stress. Journal of Agricultural and Food Chemistry, 62(25), 5897-5906.
23. Lee, M. S., Kim, I. H., Kim, C. T., & Kim, Y. (2011). Reduction of body weight by dietary garlic is associated with an increase in uncoupling protein mRNA expression and activation of AMP-activated protein kinase in diet-induced obese mice. The Journal of Nutrition, 141(11), 1947-1953.
24. Linden, A. G., Li, S., Choi, H. Y., Fang, F., Fukasawa, M., Uyeda, K., Hammer, R. E., Horton, J. D., Engelking, L. J., et al. (2018). Interplay between ChREBP and SREBP-1c coordinates postprandial glycolysis and lipogenesis in livers of mice [S]. Journal of Lipid Research, 59(3), 475-487.
25. Lindqvist, A., Baelemans, A., & Erlanson-Albertsson, C. (2008). Effects of sucrose, glucose and fructose on peripheral and central appetite signals. Regulatory Peptides, 150(1-3), 26-32.
26. Lustig, R. H. (2010). Fructose: metabolic, hedonic, and societal parallels with ethanol. Journal of the American Dietetic Association, 110(9), 1307-1321.
27. Maithilikarpagaselvi, N., Sridhar, M. G., Swaminathan, R. P., Sripradha, R., & Badhe, B. (2016). Curcumin inhibits hyperlipidemia and hepatic fat accumulation in high-fructose-fed male Wistar rats. Pharmaceutical Biology, 54(12), 2857-2863.
28. Malik, V. S., Li, Y., Pan, A., De Koning, L., Schernhammer, E., Willett, W. C., & Hu, F. B. (2019). Long-term consumption of sugar-sweetened and artificially sweetened beverages and risk of mortality in US adults. Circulation, 139(18), 2113-2125.
29. Maslov, L. N., Naryzhnaya, N. V., Boshchenko, A. A., Popov, S. V., Ivanov, V. V., & Oeltgen, P. R. (2019). Is oxidative stress of adipocytes a cause or a consequence of the metabolic syndrome? Journal of Clinical & Translational Endocrinology, 15, 1-5.
30. McMurray, F., Patten, D. A., & Harper, M. E. (2016). Reactive oxygen species and oxidative stress in obesity—recent findings and empirical approaches. Obesity, 24(11), 2301-2310.
31. Mohammadi, A., & Oshaghi, E. A. (2014). Effect of garlic on lipid profile and expression of LXR alpha in intestine and liver of hypercholesterolemic mice. Journal of Diabetes & Metabolic Disorders, 13, 20.
32. Na, L. X., Zhang, Y. L., Li, Y., Liu, L. Y., Li, R., Kong, T., & Sun, C. H. (2011). Curcumin improves insulin resistance in skeletal muscle of rats. Nutrition, Metabolism and Cardiovascular Diseases, 21(7), 526-533.
33. Nguyen, N. T., Magno, C. P., Lane, K. T., Hinojosa, M. W., & Lane, J. S. (2008). Association of hypertension, diabetes, dyslipidemia, and metabolic syndrome with obesity: findings from the National Health and Nutrition Examination Survey, 1999 to 2004. Journal of the American College of Surgeons, 207(6), 928-934.
34. Oi, Y., Kawada, T., Shishido, C., Wada, K., Kominato, Y., Nishimura, S., Ariga, T., & Iwai, K. (1999). Allyl-containing sulfides in garlic increase uncoupling protein content in brown adipose tissue, and noradrenaline and adrenaline secretion in rats. The Journal of Nutrition, 129(2), 336-342.
35. Panyod, S., & Sheen, L. Y. (2020). Beneficial effects of Chinese herbs in the treatment of fatty liver diseases. Journal of Traditional and Complementary Medicine, 10(3), 260-267.
36. Pekgor, S., Duran, C., Berberoglu, U., & Eryilmaz, M. A. (2019). The role of visceral adiposity index levels in predicting the presence of metabolic syndrome and insulin resistance in overweight and obese patients. Metabolic Syndrome and Related Disorders, 17(5), 296-302.
37. Qin, S., Huang, L., Gong, J., Shen, S., Huang, J., Ren, H., & Hu, H. (2017). Efficacy and safety of turmeric and curcumin in lowering blood lipid levels in patients with cardiovascular risk factors: a meta-analysis of randomized controlled trials. Nutrition Journal, 16, 68.
38. Rai, S. K., Sharma, M., & Tiwari, M. (2009). Inhibitory effect of novel diallyldisulfide analogs on HMG-CoA reductase expression in hypercholesterolemic rats: CREB as a potential upstream target. Life Sciences, 85(5-6), 211-219.
39. Ramos, V. W., Batista, L. O., & Albuquerque, K. T. (2017). Effects of fructose consumption on food intake and biochemical and body parameters in Wistar rats. Revista Portuguesa de Cardiologia (English Edition), 36(12), 937-941.
40. Rashid, S., Watanabe, T., Sakaue, T., & Lewis, G. F. (2003). Mechanisms of HDL lowering in insulin resistant, hypertriglyceridemic states: the combined effect of HDL triglyceride enrichment and elevated hepatic lipase activity. Clinical Biochemistry, 36(6), 421-429.
41. Rehman, K., & Akash, M. S. H. (2016). Mechanisms of inflammatory responses and development of insulin resistance: how are they interlinked? Journal of Biomedical Science, 23, 87.
42. Rodriguez-Cano, A., Mier-Cabrera, J., Balas-Nakash, M., Muñoz-Manrique, C., Legorreta-Legorreta, J., & Perichart-Perera, O. (2015). Dietary changes associated with improvement of metabolic syndrome components in postmenopausal women receiving two different nutrition interventions. Menopause (New York, NY), 22(7), 758-764.
43. Sahebkar, A., Chew, G. T., & Watts, G. F. (2014). Recent advances in pharmacotherapy for hypertriglyceridemia. Progress in Lipid Research, 56, 47-66.
44. Samuel, V. T. (2011). Fructose induced lipogenesis: from sugar to fat to insulin resistance. Trends in Endocrinology & Metabolism, 22(2), 60-65.
45. Samuel, V. T., & Shulman, G. I. (2016). The pathogenesis of insulin resistance: integrating signaling pathways and substrate flux. The Journal of Clinical Investigation, 126(1), 12-22.
46. Softic, S., Gupta, M. K., Wang, G. X., Fujisaka, S., O’Neill, B. T., Rao, T. N., Willoughby, J., Harbison, C., Fitzgerald, K., et al. (2017). Divergent effects of glucose and fructose on hepatic lipogenesis and insulin signaling. The Journal of Clinical Investigation, 127(11), 4059-4074.
47. Sperling, L. S., Mechanick, J. I., Neeland, I. J., Herrick, C. J., Després, J. P., Ndumele, C. E., Vijayaraghavan, K., Handelsman, Y., Puckrein, G. A., et al. (2015). The cardiometabolic health alliance: working toward a new care model for the metabolic syndrome. Journal of the American College of Cardiology, 66(9), 1050-1067.
48. Taskinen, M. R., Söderlund, S., Bogl, L., Hakkarainen, A., Matikainen, N., Pietiläinen, K., Räsänen, S., Lundbom, N., Björnson, E., et al. (2017). Adverse effects of fructose on cardiometabolic risk factors and hepatic lipid metabolism in subjects with abdominal obesity. Journal of Internal Medicine, 282(2), 187-201.
49. Teff, K. L., Elliott, S. S., Tschöp, M., Kieffer, T. J., Rader, D., Heiman, M., Townsend, R. R., Keim, N. L., D’alessio, D., et al. (2004). Dietary fructose reduces circulating insulin and leptin, attenuates postprandial suppression of ghrelin, and increases triglycerides in women. The Journal of Clinical Endocrinology & Metabolism, 89(6), 2963-2972.
50. Tian, Y., Su, L., Wang, J., Duan, X., & Jiang, X. (2018). Fruit and vegetable consumption and risk of the metabolic syndrome: A meta-analysis. Public Health Nutrition, 21(4), 756-765.
51. Tune, J. D., Goodwill, A. G., Sassoon, D. J., & Mather, K. J. (2017). Cardiovascular consequences of metabolic syndrome. Translational Research, 183, 57-70.
52. Vafaeipour, Z., Razavi, B. M., & Hosseinzadeh, H. (2022). Effects of turmeric (Curcuma longa) and its constituent (curcumin) on the metabolic syndrome: An updated review. Journal of Integrative Medicine, 20(3), 193-203.
53. Wu, L. Y., Chen, C. W., Chen, L. K., Chou, H. Y., Chang, C. L., & Juan, C. C. (2019). Curcumin attenuates adipogenesis by inducing preadipocyte apoptosis and inhibiting adipocyte differentiation. Nutrients, 11(10), 2307.
54. Ye, J., & Gimble, J. M. (2011). Regulation of stem cell differentiation in adipose tissue by chronic inflammation. Clinical and Experimental Pharmacology and Physiology, 38(12), 872-878.
55. Yin, J., Li, M., Xu, L., Wang, Y., Cheng, H., Zhao, X., & Mi, J. (2013). Insulin resistance determined by Homeostasis Model Assessment (HOMA) and associations with metabolic syndrome among Chinese children and teenagers. Diabetology & Metabolic Syndrome, 5, 71.

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