Antioxidant potential of Drosera peltata in Dalton Ascites Lymphoma (DAL) bearing mice


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Authors

DOI:

https://doi.org/10.29228/ijpbp.14

Keywords:

Drosera peltata, Dalton Ascites Lymphoma, Glutathione, Malondialdehyde

Abstract

Cancer is one of the prominent causes of death reported by World Health Organization (WHO). The purpose of this study was to measure the antioxidant status of animals treated with 250 and 500 mg/kg doses of ethanol and aqueous extract of Drosera peltata on Dalton Ascites Lymphoma (DAL) inoculated mice. A total of 70 mice were divided into 7 groups, each group with ten mice. The first group (negative control) received normal food and water for 14 days and was kept under normal conditions. The second group also received normal food and water for 14 days, which was used as a cancer (positive) control. The third group received 5-fluorouracil (20 mg/kg, i.p.) once a day for 14 days. The fourth and fifth group animals received 250 and 500 mg/kg of ethanol extracts of D. peltata (EEDP) whereas the sixth and seventh groups of mice received 250 and 500 mg/kg of aqueous extracts of D. peltata (AEDP), orally for 14 days. All the groups were inoculated with DAL (2 × 106 cells/mouse, i.p.) except group I, 24 hours before the commencement of the drug treatment. After the completion of treatment, blood was drawn retro-orbitally and the animals were sacrificed to isolate the liver, lungs, kidneys, and brain for observing tissue antioxidant status. The parameters analyzed were total protein (TP), catalase (CAT), malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (P), and glutathione (GSH) from the tissues apart, and the protein carbonyl content (PCC) also measured from the blood sample. Treatment with EEDP and AEDP significantly lowered the MDA levels from 23 to 10 mmol/ml in the blood, whereas from 28 to 4 nm/g in tissue isolates of the liver, lungs, kidneys, and brain. It also raised the TP, GSH, SOD, CAT, and P levels in the blood and in the tissue samples of the cancer cell line inoculated animals, where their levels were close to those observed in control (negative) group animals. The results proposed that both extracts of D. peltata ameliorated various tissue antioxidant levels in mice with DAL cancer lines comparable to the negative control.

References

Ahmad, R., Tripathi, A. K., Tripathi, P., Singh, S., Singh, R., & Singh, R. K. (2008). Malondialdehyde and protein carbonyl as biomarkers for oxidative stress and disease progression in patients with chronic myeloid leukemia. in vivo, 22(4), 525-528.

Asirvatam, R., & Christina, A. J. M. (2018). Free radical scavenging potential of Drosera indica L in presence of Dalton Ascites lymphoma (DAL) tumor bearing mice. Indonesian Journal of Pharmacy, 29(3), 127-135.

Asirvatham, R., Christina, A. J. M., & Murali, A. (2013). In vitro antioxidant and anticancer activity studies on Drosera indica L. (Droseraceae). Advanced Pharmaceutical Bulletin, 3(1), 115-120.

Asirvatham, R., Mathew, A. A., & Dawn, V. T. (2020). In vivo antianemic study of two species of Murva on phenylhydrazine induced anaemia in rats. Adıyaman Üniversitesi Sağlık Bilimleri Dergisi, 6(2), 243-247.

Balaji, V. R., & Asirvatham, R. (2015). In vitro anticancer and antioxidant activity studies on Drosera peltata JE Sm. Spatula DD, 5(3), 183-199.

Baskar, R., Lee, K. A., Yeo, R., & Yeoh, K. W. (2012). Cancer and radiation therapy: current advances and future directions. International Journal of Medical Sciences, 9(3), 193-199.

Christina, A., Joseph, D. G., Packialakshmi, M., Kothai, R., Robert, S. J. H., Chidambaranathan, N., & Ramasamy, M. (2004). Anticarcinogenic activity of Withania somnifera Dunal against Dalton’s ascitic lymphoma. Journal of Ethnopharmacology, 93(2-3), 359-361.

Demirci, S., Ozsaran, Z., Celik, H. A., Aras, A. B., & Aydin, H. H. (2011). The interaction between antioxidant status and cervical cancer: a case control study. Tumori Journal, 97(3), 290-295.

Desai, A. G., Qazi, G. N., Ganju, R. K., El-Tamer, M., Singh, J., Saxena, A. K., Bedi, Y. S., Taneja, S. C., & Bhat, H. K. (2008). Medicinal plants and cancer chemoprevention. Current Drug Metabolism, 9(7), 581-591.

DeWys, W. D. (1982). Pathophysiology of cancer cachexia: current understanding and areas for future research. Cancer Research, 42(2_Supplement), 721-725.

Gupta, M., Mazumder, U. K., Kumar, R. S., Sivakumar, T., & Vamsi, M. L. M. (2004). Antitumor activity and antioxidant status of Caesalpinia bonducella against Ehrlich ascites carcinoma in Swiss albino mice. Journal of Pharmacological Sciences, 94(2), 177-184.

Ichimura, Y., Habuchi, T., Tsuchiya, N., Wang, L., Oyama, C., Sato, K., Nishiyama, H., Ogawa, O., & Kato, T. (2004). Increased risk of bladder cancer associated with a glutathione peroxidase 1 codon 198 variant. The Journal of Urology, 172(2), 728-732.

Koca, R., Armutcu, F., Altinyazar, C., & Gürel, A. (2005). Evaluation of lipid peroxidation, oxidant/antioxidant status, and serum nitric oxide levels in Alopecia areata. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 11(6), 296-299.

Levine, R. L., Wehr, N., Williams, J. A., Stadtman, E. R., & Shacter, E. (2000). Determination of carbonyl groups in oxidized proteins. Stress Response, 99, 15-24.

Liu, M., Pelling, J. C., Ju, J., Chu, E., & Brash, D. E. (1998). Antioxidant action via p53-mediated apoptosis. Cancer Research, 58(8), 1723-1729.

Maity, P., Chakraborty, S., & Bhattacharya, P. (2000). Neovascularisation offers a new perspective to glutamine related therapy. Indian Journal of Experimental Biology, 38(1), 88-90.

Newman, D. J., & Cragg, G. M. (2009). Natural product scaffolds as leads to drugs. Future Medicinal Chemistry, 1(8), 1415-1427.

Parasuraman, S., Thing, G. S., & Dhanaraj, S. A. (2014). Polyherbal formulation: Concept of ayurveda. Pharmacognosy Reviews, 8(16), 73-80.

Rajesh, M., Sulochana, K. N., Coral, K., Punitham, R., Biswas, J., Babu, K., & Ramakrishnan, S. (2004). Determination of carbonyl group content in plasma proteins as a useful marker to assess impairment in antioxidant defense in patients with Eales' disease. Indian Journal of Ophthalmology, 52(2), 139-144.

Raju, A., Christina, M., & Murali, A. (2012). Antitumor activity of ethanol and aqueous extracts of Drosera burmannii vahl. in EAC bearing mice. Spatula DD, 2(2), 83-88.

Raju, A., Salwa, A. S., Daisy, P. A., Aparna, A. M., & Boby, J. (2022). Neuroprotective effect of Vanda tessellata as “Rasna” Species, on aluminium chloride induced Alzheimer's in rats: Neuroprotective study of Rasna. Journal of Microbiology, Biotechnology and Food Sciences, 12(1), e5164.

Reddy, C. S., Reddy, K., & Jadhav, S. (2001). Threatened (medicinal) plants of Andhra Pradesh. Medicinal Plants Conservation Center, 1-39.

Ruby, A. J., Kuttan, G., Babu, K. D., Rajasekharan, K., & Kuttan, R. (1995). Anti-tumour and antioxidant activity of natural curcuminoids. Cancer Letters, 94(1), 79-83.

Sara, J., Abdolrasoul, E., Ahmad, K., & Hossain, B. (2015). Evaluation of antioxidant activity of Malus domestica fruit extract from Kashan area. African Journal of Agricultural Research, 10(20), 2136-2140.

Shaik, I. H., & Mehvar, R. (2006). Rapid determination of reduced and oxidized glutathione levels using a new thiol-masking reagent and the enzymatic recycling method: application to the rat liver and bile samples. Analytical and Bioanalytical Chemistry, 385(1), 105-113.

Sun, Y., Oberley, L. W., Elwell, J. H., & Sierra‐Rivera, E. (1989). Antioxidant enzyme activities in normal and transformed mouse liver cells. International Journal of Cancer, 44(6), 1028-1033.

Vani, M., Reddy, G. P., Reddy, G. R., Thyagaraju, K., & Reddanna, P. (1990). Glutathione-S-transferase, superoxide dismutase, xanthine oxidase, catalase, glutathione peroxidase and lipid peroxidation in the liver of exercised rats. Biochemistry International, 21(1), 17-26.

WHO (World Health Organization). (2022). Cancer. Retrieved from https://www.who.int/news-room/fact-sheets/detail/cancer. Accessed 3 February 2022.

Yagi, K. (1987). Lipid peroxides and human diseases. Chemistry and Physics of Lipids, 45(2-4), 337-351.

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Published

14.10.2022

How to Cite

Asirvatham, R., & Christina, A. J. M. (2022). Antioxidant potential of Drosera peltata in Dalton Ascites Lymphoma (DAL) bearing mice. International Journal of Plant Based Pharmaceuticals, 3(1), 41–46. https://doi.org/10.29228/ijpbp.14

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Research Articles
Received 2022-09-19
Accepted 2022-10-10
Published 2022-10-14