FTIR spectral correlation with alpha-glucosidase inhibitory activities of selected leafy plants extracts
Abstract views: 146 / PDF downloads: 89
Keywords:Leafy plants, FTIR, PLS regression analysis, Alpha-glucosidase inhibitory activity
Fourier transform infrared spectroscopy (FTIR) is a simple, rapid analytical technique used for the identification of organic functional groups of biomolecules. This study aimed to investigate the use of FTIR spectroscopy method for rapid detection of the α-glucosidase inhibitory activity of crude extracts of edible leafy plants, characterization of functional groups of chemical components present in crude extracts, and identification of possible biomolecules responsible for α-glucosidase inhibitory activity. Powdered leaves of five different plants, namely Le-kola pala (LE) (Premna procumbens), Kora kaha (KK) (Memecylon umbellatum), Koppa (KO) (Polyscias scutellaria), Stevia (ST) (Stevia rebaudiana), and Yaki naran (YK) (Atlantia ceylanica) were sequentially extracted with hexane, ethyl acetate (EtOAc) and methanol (MeOH). The FTIR spectra of crude plant extracts were obtained following the KBr pellet method, within the range of 4000-500 cm-1. The plant extracts were subjected to assay the α-glucosidase inhibitory activity. Further, the multivariate predictive models for α-glucosidase inhibitory activity were developed using partial least square (PLS) regression analysis. The highest Rc2 (0.96), Rcv2 (0.87), Rp2 (0.93), and the lowest RMSEC (24.10), RMSECV (41.70), and RMSEP (81.04) values were noticed for spectral region range from 1700 cm-1 to 1800 cm-1, indicating the strongest correlation to the α-glucosidase inhibitory activity, while the spectral region range from 1500 cm-1 to 1700 cm-1 was found to have the lowest Rc2 (0.71), Rcv2 (0.52), Rp2 (0.45) and the highest RMSEC (61.14) and RMSECV (80.21), indicating the lowest correlation to the α-glucosidase inhibitory activity. As the peak appearing in the range of 1700-1800 cm-1 is usually ascribed to C=O stretching vibration of ester groups, ketones, and carboxylic acids, there was a strong correlation between α-glucosidase inhibitory activity with those organic functional groups. The present study suggests that FTIR spectral analysis together with PLS regression analysis would be a convenient, rapid tool to determine α-glucosidase inhibitory activity of plant extracts.
Adina, C., Florinela, F., Abdelmoumen, T., & Carmen, S. (2010). Application of FTIR spectroscopy for a rapid determination of some hydrolytic enzymes activity on sea buckthorn substrate. Romanian Biotechnological Letters, 15(6), 5738-5744.
Ashokkumar, R., & Ramaswamy, M. (2014). Phytochemical screening by FTIR spectroscopic analysis of leaf extracts of selected Indian medicinal plants. International Journal of Current Microbiology and Applied Sciences, 3(1), 395-406.
Bhardwaj, M., Yadav, P., Dalal, S., & Kataria, S. K. (2020). A review on ameliorative green nanotechnological approaches in diabetes management. Biomedicine & Pharmacotherapy, 127, 110198.
Bunaciu, A. A., Aboul-Enein, H. Y., & Fleschin, S. (2011). Recent applications of fourier transform infrared spectrophotometry in herbal medicine analysis. Applied Spectroscopy Reviews, 46(4), 251-260.
Coates, J. (2000). Interpretation of infrared spectra, a practical approach. In R. A. Meyers (Ed.), Encyclopedia of Analytical Chemistry: US: John Wiley & Sons, Ltd.
De Souza Schmidt Goncalves, A. E., Lajolo, F. M., & Genovese, M. I. (2010). Chemical composition and antioxidant/antidiabetic potential of Brazilian native fruits and commercial frozen pulps. Journal of Agricultural and Food Chemistry, 58(8), 4666-4674.
Easmin, S., Sarker, M. Z. I., Ghafoor, K., Ferdosh, S., Jaffri, J., Ali, M. E., Mirhosseini, H., Al-Juhaimi, F. Y., Perumal, V., et al. (2017). Rapid investigation of α-glucosidase inhibitory activity of Phaleria macrocarpa extracts using FTIR-ATR based fingerprinting. Journal of Food and Drug Analysis, 25(2), 306-315.
Ediriweera, E., & Ratnasooriya, W. (2009). A review on herbs used in treatment of diabetes mellitus by Sri Lankan ayurvedic and traditional physicians. Ayu, 30(4), 373-391.
Ferreira, P. S., Victorelli, F. D., Fonseca-Santos, B., & Chorilli, M. (2019). A review of analytical methods for p-coumaric acid in plant-based products, beverages, and biological matrices. Critical Reviews in Analytical Chemistry, 49(1), 21-31.
Gunarathne, R., Marikkar, N., Mendis, E., Yalegama, C., Jayasinghe, L., Liyanage, R., & Jayaweera, S. (2022a). Bioactivity studies of different solvent extracts of partially defatted coconut testa obtained from selected coconut cultivars. The Journal of Agricultural Sciences-Sri Lanka, 17(1), 171-184.
Gunarathne, R., Marikkar, N., Mendis, E., Yalegama, C., Jayasinghe, L., & Ulpathakumbura, S. (2022b). Mid-IR Spectral Characterization and Chemometric Evaluation of Different Solvent Extracts of Coconut Testa Flour. Journal of Food Chemistry and Nanotechnology, 8(3), 69-75.
Gunarathne, R., Marikkar, N., Yalegama, C., & Mendis, E. (2022c). FTIR spectral analysis combined with chemometrics in evaluation of composite mixtures of coconut testa flour and wheat flour. Journal of Food Measurement and Characterization, 16(3), 1796-1806.
Hari, N., & Nair, V. P. (2018). FTIR spectroscopic analysis of leaf extract in hexane in Jasminum azoricum L. Recent Research in Science and Technology, 4(8), 170-172.
Jayaraj, S., Suresh, S., & Kadeppagari, R. K. (2013). Amylase inhibitors and their biomedical applications. Starch‐Stärke, 65(7‐8), 535-542.
Kazeem, M., Adamson, J., & Ogunwande, I. (2013). Modes of inhibition of α-amylase and α-glucosidase by aqueous extract of Morinda lucida Benth leaf. BioMed Research International, 2013, 527570.
Mancuso, C., & Santangelo, R. (2014). Ferulic acid: Pharmacological and toxicological aspects. Food and Chemical Toxicology, 65, 185-195.
Mittal, P., Goswami, M., & Airi, M. (2020). Phytochemical, FTIR and NMR Analysis of Crude Extract of Duranta plumieri leaves. Journal of Pharmaceutical Sciences and Research, 12(1), 182-185.
Nandiyanto, A. B. D., Oktiani, R., & Ragadhita, R. (2019). How to read and interpret FTIR spectroscope of organic material. Indonesian Journal of Science and Technology, 4(1), 97-118.
Nickavar, B., & Abolhasani, L. (2013). Bioactivity-guided separation of an α-amylase inhibitor flavonoid from Salvia virgata. Iranian Journal of Pharmaceutical Research, 12(1), 57-61.
Nipun, T. S., Khatib, A., Ahmed, Q. U., Redzwan, I. E., Ibrahim, Z., Khan, A. a. Y. F., Primaharinastiti, R., Khalifa, S. A., & El-Seedi, H. R. (2020). Alpha-glucosidase inhibitory effect of Psychotria malayana jack leaf: A rapid analysis using infrared fingerprinting. Molecules, 25(18), 4161.
Nokhala, A., Ahmed, Q. U., Saleh, M. S., Nipun, T. S., Khan, A. A. Y. F., & Siddiqui, M. J. (2020). Characterization of α-glucosidase inhibitory activity of Tetracera scandens leaves by fourier transform infrared spectroscopy-based metabolomics. Advances in Traditional Medicine, 20, 169-180.
Pandi, A., & Kalappan, V. M. (2021). Pharmacological and therapeutic applications of Sinapic acid—An updated review. Molecular Biology Reports, 48(4), 3733-3745.
Saleh, M. S., Siddiqui, M. J., Mat So’ad, S. Z., Roheem, F. O., Saidi-Besbes, S., & Khatib, A. (2018). Correlation of FT-IR fingerprint and α-glucosidase inhibitory activity of salak (Salacca zalacca) fruit extracts utilizing orthogonal partial least square. Molecules, 23(6), 1434.
Shobana, S., Sreerama, Y., & Malleshi, N. (2009). Composition and enzyme inhibitory properties of finger millet (Eleusine coracana L.) seed coat phenolics: Mode of inhibition of α-glucosidase and pancreatic amylase. Food Chemistry, 115(4), 1268-1273.
Ulpathakumbura, S., Marikkar, N., & Jayasinghe, L. (2023). Anti-oxidative, anti-hyperglycemic and anti-obesity properties of selected edible leafy plants of Sri Lanka. Food Chemistry Advances, 2, 100208.
Umar, A. H., Ratnadewi, D., Rafi, M., & Sulistyaningsih, Y. C. (2021). Untargeted metabolomics analysis using FTIR and UHPLC-Q-Orbitrap HRMS of two Curculigo species and evaluation of their antioxidant and α-glucosidase inhibitory activities. Metabolites, 11(1), 42.
Vermerris, W., & Nicholson, R. (2008). Families of Phenolic Compounds and Means of Classification. In W. Vermerris & R. Nicholson (Eds.), Phenolic Compound Biochemistry (pp. 1-34): Springer, Dordrecht.
White, J. L. (1971). Interpretation of infrared spectra of soil minerals. Soil Science, 112(1), 22-31.
Xiao-Ping, Y., Chun-Qing, S., Ping, Y., & Ren-Gang, M. (2010). α-Glucosidase and α-amylase inhibitory activity of common constituents from traditional Chinese medicine used for diabetes mellitus. Chinese Journal of Natural Medicines, 8(5), 349-352.
How to Cite
Copyright (c) 2023 Savani Ulpathakumbura, Nazrim Marikkar, Lalith Jayasinghe
This work is licensed under a Creative Commons Attribution 4.0 International License.
The papers published in the International Journal of Plant Based Pharmaceuticals are licenced under Creative Commons Attribution 4.0 International Licence (CC BY).