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International Journal of Plant Based Pharmaceuticals
e‑ISSN 2791-7509 Open Access · CC BY 4.0
Vol. 3 · Issue 2 · 2023 Dec 12, 2023 Research Articles

Assessment of the analgesic potential of ethylacetate leaf fraction of Sida linifolia L. (Malvaceae)

EE
Emmanuel Chimeh Ezeako Corresponding University of Nigeria, Department of Biochemistry, Nsukka, Enugu, Nigeria ezeako.emmanuel.188647@unn.edu.ng Nigeria
AS
Abel Yashim Solomon Nile University, College of Health Sciences, Department of Anatomy, Abuja, Nigeria Nigeria
HN
Hope Chimbuezie Ngozi University of Nigeria, Department of Microbiology, Nsukka, Enugu, Nigeria Nigeria
AA
Abayomi Oyeyemi Ajagbe Nile University, College of Health Sciences, Department of Anatomy, Abuja, Nigeria Nigeria
LA
Linus Ogbonnaya Agwu Federal University of Technology, Owerri, Imo, Nigeria Nigeria
LM
Lomi Oruchukwu Michael University of Nigeria, Department of Biochemistry, Nsukka, Enugu, Nigeria Nigeria
EO
Evaristus Chinonso Odoh Modibbo Adama University Teaching Hospital, Department of Pharmacy, Yola, Adamawa, Nigeria Nigeria
VO
Victor Inioluwa Olaoye University of Ibadan, Department of Chemistry, Ibadan, Nigeria Nigeria
PA
Philip Nwachukwu Ashiakpa University of Nigeria, Department of Biochemistry, Nsukka, Enugu, Nigeria Nigeria
MN
Martin Benedict Nworie University of Nigeria, Department of Biochemistry, Nsukka, Enugu, Nigeria Nigeria
VO
Vincent E. Ozougwu University of Nigeria, Department of Biochemistry, Nsukka, Enugu, Nigeria Nigeria
PJ
Parker Elijah Joshua University of Nigeria, Department of Biochemistry, Nsukka, Enugu, Nigeria Nigeria
Pages228-239 PublishedDec 12, 2023 LicenseOpen Access
Full Text
IJPBP 2 VOL 3 · 2
VOL 3 · ISS 2 · 2023 View issue

Abstract

Sida linifolia L. is a valuable plant of West Tropical Africa with several folklore claims and growing evidence of its bioactivity, including its pain-relieving potential; however, scientific validation of these claims is still limited. This study investigated the phytochemical composition and analgesic potential of the ethyl acetate fraction of the hydro-alcoholic extract of S. linifolia leaves (SEAL). Gas chromatography-flame ionization detector (GC-FID) and high-performance liquid chromatography (HPLC) techniques were used to determine the phytochemistry of SEAL. Formalin and acetic acid models were employed to determine the analgesic properties of SEAL. The result of GC-FID analysis revealed varying concentrations of lunamarine, naringenin, ephedrine, catechins, cardiac glycosides, flavanones, kaempferol, flavones, phytate, rutin, steroids, tannins, oxalate, flavan-3-ols, sapogenins, cyanogenic glycosides, anthocyanin, and proanthocyanidins in SEAL. In addition, varying concentrations of polyphenolics, such as phenylacetic acid, caffeic acid, ellagic acid, and naringenin were detected in SEAL via HPLC analysis. The LD50 study showed that SEAL was safe up to 5000 mg/kg body weight per oral (p.o.) in Swiss mice. Pretreatment with oral doses (200, 400, and 600 mg/kg bw) of SEAL significantly (p < 0.05) inhibited all phases of formalin-induced hind paw licking and acetic acid-induced writhing syndrome in mice compared to positive control and was on par with aspirin (100 mg/kg bw p.o.). The observed bioactivity of SEAL could be anchored to its phytoconstituents. Therefore, the plant leaf fraction represents a good source of bioactive compounds with immense potential for exploration in therapeutic research.

Keywords

#Sida linifolia #Phytocompounds #Polyphenolics #Analgesic #Medicinal plant

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Copyright (c) 2023 Emmanuel Chimeh Ezeako, Abel Yashim Solomon, Hope Chimbuezie Ngozi, Abayomi Oyeyemi Ajagbe, Linus Ogbonnaya Agwu, Lomi Oruchukwu Michael, Evaristus Chinonso Odoh, Victor Inioluwa Olaoye, Philip Nwachukwu Ashiakpa, Martin Benedict Nworie, Vincent E. Ozougwu, Parker Elijah Joshua

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