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International Journal of Plant Based Pharmaceuticals
e‑ISSN 2791-7509 Open Access · CC BY 4.0
Vol. 2 · Issue 1 · 2022 Dec 31, 2021 Research Articles

Effects of systemic hydroxytyrosol application in experimental periodontitis of rats

MS
Mehmet Cihan Sengun Bolu Abant Izzet Baysal University, Faculty of Dentistry, Department of Periodontology, Bolu, Turkey Turkey
SG
Sadiye Gunpinar Corresponding Bolu Abant Izzet Baysal University, Faculty of Dentistry, Department of Periodontology, Bolu, Turkey coskunersadiye@gmail.com Turkey
Pages42-50 PublishedDec 31, 2021 LicenseOpen Access
Full Text
IJPBP 1 VOL 2 · 1
VOL 2 · ISS 1 · 2022 View issue

Abstract

This study aimed to determine the effects of systemically administered hydroxytyrosol (HT) on alveolar bone resorption and oxidative stress parameters in experimental periodontitis (EP). Thirty-two rats were divided randomly into four groups; 1) periodontally healthy + serum physiologic (PH-SP), 2) PH + hydroxytyrosol (PH-HT), 3) experimental periodontitis + SP (EP-SP), and 4) EP-HT. Following induction of EP, 10 mg/kg of systemic HT (test)/SP (control) was administered and continued for 14 days. The animals were euthanized on the 15th day, and the jaws were removed for histopathologic, histomorphometric, and immunohistochemical analyses. Enzyme-linked immunosorbent assay (ELISA) was used to analyze serum and gingival tissue malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) levels. The receptor activator of NF-kB ligand (RANKL), osteoprotegerin (OPG) levels, and RANKL/OPG ratio were analyzed via immunolabeling. Serum and tissue MDA, SOD, and GSH-Px levels did not differ between the groups. The immunohistochemical evaluation showed that RANKL levels and RANKL/OPG ratio in HT applied groups were significantly lower than SP applied groups. Within the limits of this study, daily administration of hydroxytyrosol at a dose of 10 mg/kg for 14 days could prevent alveolar bone destruction in experimental periodontitis. Besides, the antioxidant effect of HT could not be revealed.

Keywords

#Alveolar bone loss #Antioxidant #Experimental periodontitis #Hydroxytyrosol #Oxidative stress

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Copyright (c) 2022 Mehmet Cihan Sengun, Sadiye Gunpinar

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