A North Cameroonian cultivar of Hibiscus sabdariffa (Malvaceae) with calyces enriched in anthocyanins

Abstract

Folere ( Hibiscus sabdariffa L.) is a traditional plant cultivated in the north of Cameroon near the city of Yagoua. Calyces are used to prepare beverages while the leaves are incorporated into meals. This Cameroonian roselle is characterized by a higher intensity of red color than that found in conspecific samples from Egypt, and it was of interest to us to evaluate its beneficial activities in comparison with European pharmacopoeia standards. The samples were first subjected to phylogenetic analyses. Five samples including samples from Egypt and a white flower variety were subsequently examined according to the European pharmacopeia criteria i.e. (i) taxonomic identification, (ii) sample preparation and characterization (desiccation, TLC, coloring power, presence of foreign matter), and (iii) determination of the acidity index. Anthocyanins and anthocyanidins were quantified in three extracts from methanolic maceration, infusion, and boiling in water. Taken together, the results confirmed that the Cameroonian variety indeed belongs to H. sabdariffa and meets European Pharmacopoeia standards. All extracts contained common anthocyanins (delphinidin-3-sambioside, delphinidin-3-glucoside, cyanidin-3-sambioside, cyanidin-3-glucoside), and delphinidin and cyanidin aglycones were also found in the organic extracts. As a result of anthocyanin quantification by HPLC in Cameroon samples, it was determined that they showed a high concentration of delphinidin type anthocyanins and a concentration 10 times higher than the Egyptian variety. This variety appears to be a very promising candidate for inclusion in industrial preparations, thanks to its high anthocyanin qualitative and quantitative properties, and also maintains economic interest for local populations.

Keywords

References

1. Agrawal, K. K. (2020). A Review on Pharmacological Validation of Genus Hibiscus with Main Emphasis on Hibiscus rosa-sinensis. International Journal of Pharmacy & Pharmaceutical Research, 18(3), 451-462.
2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403-410. https://doi.org/10.1016/S0022-2836(05)80360-2
3. ANSM Monograph. (2013). Ptisanae, Monograph 1435. In French Pharmacopeia 11ème ed.
4. Besharati, J., Shirmardi, M., Meftahizadeh, H., Ardakani, M. D., & Ghorbanpour, M. (2022). Changes in growth and quality performance of Roselle (Hibiscus sabdariffa L.) in response to soil amendments with hydrogel and compost under drought stress. South African Journal of Botany, 145, 334-347. https://doi.org/10.1016/j.sajb.2021.03.018
5. Branch, S. K. (2005). Guidelines from the international conference on harmonisation (ICH). Journal of Pharmaceutical and Biomedical Analysis, 38(5), 798-805. https://doi.org/10.1016/j.jpba.2005.02.037
6. Cisse, M., Dornier, M., Sakho, M., Ndiaye, A., Reynes, M., & Sock, O. (2009). Le bissap (Hibiscus sabdariffa L.): composition et principales utilisations. Fruits, 64(3), 179-193. https://doi.org/10.1051/fruits/2009013
7. Da-Costa-Rocha, I., Bonnlaender, B., Sievers, H., Pischel, I., & Heinrich, M. (2014). Hibiscus sabdariffa L.–A phytochemical and pharmacological review. Food Chemistry, 165, 424-443. https://doi.org/10.1016/j.foodchem.2014.05.002
8. Emmanuel, M. M., & Didier, D. S. (2011). Medicinal plant knowledge of ethnic groups in Douala town, Cameroon. American Journal of Food and Nutrition, 1(4), 178-184. https://doi.org/10.5251/ajfn.2011.1.4.178.184
9. Fokunang, C., Ndikum, V., Tabi, O., Jiofack, R., Ngameni, B., Guedje, N., Tembe-Fokunang, E., Tomkins, P., Barkwan, S., & Kechia, F. (2011). Traditional medicine: past, present and future research and development prospects and integration in the National Health System of Cameroon. African Journal of Traditional, Complementary and Alternative Medicines, 8(3), 284-295. https://doi.org/10.4314/ajtcam.v8i3.65276
10. Fongnzossie, E., Tize, Z., Nde, P. F., Biyegue, C. N., Ntsama, I. B., Dibong, S., & Nkongmeneck, B. (2017). Ethnobotany and pharmacognostic perspective of plant species used as traditional cosmetics and cosmeceuticals among the Gbaya ethnic group in Eastern Cameroon. South African Journal of Botany, 112, 29-39. https://doi.org/10.1016/j.sajb.2017.05.013
11. Fongod, A., Ngoh, L., & Veranso, M. (2014). Ethnobotany, indigenous knowledge and unconscious preservation of the environment: An evaluation of indigenous knowledge in South and Southwest Regions of Cameroon. International Journal of Biodiversity and Conservation, 6(1), 85-99. https://doi.org/10.5897/IJBC2013.0637
12. Fournier, G., Bausset, J., Maciuk, A., Thouminot, C., & Beherec, O. (2011). Influence des conditions de séchage et de conservation des échantillons de chanvre prélevés dans les cultures industrielles afin d’en déterminer réglementairement la teneur en Δ-9-THC. Paper presented at the Annales de Toxicologie Analytique. http://dx.doi.org/10.1051/ata/2011112
13. Hall, T. A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Paper presented at the Nucleic Acids Symposium Series.
14. Huelsenbeck, J. P., Larget, B., & Alfaro, M. E. (2004). Bayesian phylogenetic model selection using reversible jump Markov chain Monte Carlo. Molecular Biology and Evolution, 21(6), 1123-1133. https://doi.org/10.1093/molbev/msh123
15. Jiofack, T., Fokunang, C., Guedje, N., Kemeuze, V., Fongnzossie, E., Nkongmeneck, B. A., Mapongmetsem, P. M., & Tsabang, N. (2010). Ethnobotanical uses of medicinal plants of two ethnoecological regions of Cameroon. International Journal of Medicine and Medical Sciences, 2(3), 60-79.
16. Karkade Monograph. (2017). Karkadé, Monograph 1502. In European Pharmacopeia 9.4. Concil of Europe. Strasbourg.
17. Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547-1549. https://doi.org/10.1093/molbev/msy096
18. Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., & Higgins, D. G. (2007). Clustal W and Clustal X version 2.0. Bioinformatics, 23(21), 2947-2948. https://doi.org/10.1093/bioinformatics/btm404
19. Laskar, Y. B., & Mazumder, P. B. (2020). Insight into the molecular evidence supporting the remarkable chemotherapeutic potential of Hibiscus sabdariffa L. Biomedicine & Pharmacotherapy, 127, 110153. https://doi.org/10.1016/j.biopha.2020.110153
20. Maddison, W. P. (1997). Gene trees in species trees. Systematic Biology, 46(3), 523-536. https://doi.org/10.1093/sysbio/46.3.523
21. Maganha, E. G., da Costa Halmenschlager, R., Rosa, R. M., Henriques, J. A. P., de Paula Ramos, A. L. L., & Saffi, J. (2010). Pharmacological evidences for the extracts and secondary metabolites from plants of the genus Hibiscus. Food Chemistry, 118(1), 1-10. https://doi.org/10.1016/j.foodchem.2009.04.005
22. Najafpour Boushehri, S., Karimbeiki, R., Ghasempour, S., Ghalishourani, S. S., Pourmasoumi, M., Hadi, A., Mbabazi, M., Pour, Z. K., Assarroudi, M., & Mahmoodi, M. (2020). The efficacy of sour tea (Hibiscus sabdariffa L.) on selected cardiovascular disease risk factors: A systematic review and meta‐analysis of randomized clinical trials. Phytotherapy Research, 34(2), 329-339. https://doi.org/10.1002/ptr.6541
23. Ngatcha, B. N., Mudry, J., Wakponou, A., Ekodeck, G., Njitchoua, R., & Sarrot-Reynauld, J. (2001). Le cordon sableux Limani-Yagoua, extrême-nord Cameroun, et son rôle hydraulique. Journal of African Earth Sciences, 32(4), 889-898. https://doi.org/10.1016/S0899-5362(02)00061-1
24. Notredame, C., Higgins, D. G., & Heringa, J. (2000). T-Coffee: A novel method for fast and accurate multiple sequence alignment. Journal of Molecular Biology, 302(1), 205-217. https://doi.org/10.1006/jmbi.2000.4042
25. Ojulari, O. V., Lee, S. G., & Nam, J. O. (2019). Beneficial effects of natural bioactive compounds from Hibiscus sabdariffa L. on obesity. Molecules, 24(1), 210. https://doi.org/10.3390/molecules24010210
26. Onana, J. M. (2011). The vascular plants of Cameroon: a taxonomic checklist with IUCN assessments: National Herbarium of Cameroon.
27. Opletal, L., Chocholousova-Havlikova, L., Siatka, T., Cahliková, L., Locarek, M., Ali, B. H., Manoj, P., Ramkumar, A., Al Suleimani, Y. M., & Al Za'abi, M. (2017). Preparation and validated analysis of anthocyanin concentrate from the calyces of Hibiscus sabdariffa. Natural Product Communications, 12(1), 43-45. https://doi.org/10.1177/1934578X1701200112
28. Özdogan, F., Orhan, N., & Ergun, F. (2011). Studies on the conformity of Hibiscus sabdariffa L. samples from Turkish market to European pharmacopeia. FABAD Journal of Pharmaceutical Sciences, 36, 25-32.
29. Pamilo, P., & Nei, M. (1988). Relationships between gene trees and species trees. Molecular Biology and Evolution, 5(5), 568-583. https://doi.org/10.1093/oxfordjournals.molbev.a040517
30. Raghu, K., Naidoo, Y., & Dewir, Y. (2019). Secretory structures in the leaves of Hibiscus sabdariffa L. South African Journal of Botany, 121, 16-25. https://doi.org/10.1016/j.sajb.2018.08.018
31. Riaz, G., & Chopra, R. (2018). A review on phytochemistry and therapeutic uses of Hibiscus sabdariffa L. Biomedicine & Pharmacotherapy, 102, 575-586. https://doi.org/10.1016/j.biopha.2018.03.023
32. Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D. L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M. A., & Huelsenbeck, J. P. (2012). MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61(3), 539-542. https://doi.org/10.1093/sysbio/sys029
33. Schütz, K., Persike, M., Carle, R., & Schieber, A. (2006). Characterization and quantification of anthocyanins in selected artichoke (Cynara scolymus L.) cultivars by HPLC–DAD–ESI–MS. Analytical and Bioanalytical Chemistry, 384, 1511-1517. https://doi.org/10.1007/s00216-006-0316-6
34. Vasudeva, N., & Sharma, S. (2008). Biologically Active Compounds from the Genus Hibiscus. Pharmaceutical Biology, 46(3), 145-153. https://doi.org/10.1080/13880200701575320
35. Veluru, B., Kumar, R., Shivashankara, K. S., Bharathi, T. U., Rao, T., Sane, A., Roy, T. K., & Rao, D. D. K. (2022). Anthocyanin profile diversity in China aster (Callistephus chinensis L. Nees) genotypes. South African Journal of Botany, 151, 107-113. https://doi.org/10.1016/j.sajb.2021.11.047
36. Werner, O., Magdy, M., & Ros, R. M. (2016). Molecular systematics of Abelmoschus (Malvaceae) and genetic diversity within the cultivated species of this genus based on nuclear ITS and chloroplast rpL16 sequence data. Genetic Resources and Crop Evolution, 63, 429-445. https://doi.org/10.1007/s10722-015-0259-x
37. Wilson, F. D. (2006). A distributional and cytological survey of the presently recognized taxa of Hibiscus section Furcaria (Malvaceae). Bonplandia, 15(1-2), 53-62.

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Copyright (c) 2024 Raynatou Ba Ibrahim, Sarah Orion, Olaf Werner, Pauline Gautier, Brigitte Njikeuntchi–Bureau, Loic Bureau, Brice Autier, Marylène Chollet-Krugler, Françoise Lohézic–Le Dévéhat