Antiurolithiatic activity of Indian medicinal plant: Ocimum kilimandscharicum Gurke (Lamiaceae)

Prathibha Guttal Subhas; Gangadharaih Narayana Murthy; Nabarun Mukhopadhyay

doi:10.62313/ijpbp.2024.203

Authors

Prathibha Guttal Subhas National College of Pharmacy, Department of Pharmacognosy, Shimoga 577201, Karnataka, India https://orcid.org/0009-0000-0107-3285
Gangadharaih Narayana Murthy National College of Pharmacy, Department of Pharmacognosy, Shimoga 577201, Karnataka, India https://orcid.org/0009-0006-4917-0889
Nabarun Mukhopadhyay Bapuji Pharmacy College, Department of Pharmacognosy, Davanagere 577004, Karnataka, India https://orcid.org/0000-0002-8851-9485

DOI:

https://doi.org/10.62313/ijpbp.2024.203

Keywords:

Antiurolithiatic, Calcium oxalate crystals, Cystone, In vitro antiurolithiatic activity, Ocimum kilimandscharicum

Abstract

Urolithiasis is the most prevalent condition of the urinary system, characterized by the formation of stones inside the urinary tract. It is urgent to look for a natural urolithiasis therapy due to the serious side effects of conventional medications. Hydro-alcoholic (80% v/v) extract of the aerial parts of Ocimum kilimandscharicum (OK) and its ethyl acetate, chloroform, n-butanol, aqueous, and n-hexane fractions were subjected to in vitro antiurolithiatic screening as well as preliminary screening of phytochemicals. The in vitro antiurolithiatic activity of O. kilimandscharicum was studied using its hydroalcoholic extract (HAEOK). Calcium phosphate test using a colorimetric approach and calcium oxalate assay using a titrimetric model were used to determine the proportion of calcium oxalate crystals that dissolved. Total phenolic content (TPC) and total flavonoid content (TFC) were measured for the extract and fractions of OK. Ethyl acetate fraction (EAFOK) had a greater capacity to suppress crystal formation in both the calcium phosphate and calcium oxalate assays. The percent dissolution of calcium oxalate by HAEOK and EAFOK (31.48 ± 0.920% and 39.21 ± 0.903%) and calcium phosphate crystals by HAEOK and EAFOK (59.03 ± 0.820% and 66.62 ± 0.468%) was determined, respectively. At p < 0.05 and p < 0.01, differences between the results were regarded as significant. Cystone was employed as a standard drug. This study revealed that EAFOK showed significant antiurolithiatic activity. The antiurolithiatic activity of the extract/fraction was attributed to the steroids, triterpenoids, and flavonoid content of OK.

References

Ahmad, E., Jahangir, M., Bukhari, N. I., Sarwar, A., Aziz, T., Alharbi, M., Alsahammari, A., & Alasmari, A. F. (2023). Isolation, structure elucidation & antidiabetic potential of Rosa brunonii L. fruit–fight diabetes with natural remedies. Journal of the Chilean Chemical Society, 68(2), 5887-5894. http://dx.doi.org/10.4067/s0717-97072023000205887 DOI: https://doi.org/10.4067/s0717-97072023000205887

Ammara, A., Sobia, A., Nureen, Z., Sohail, A., Abid, S., Aziz, T., Nahaa, M., Rewaa, S., Ahellah, M., & Nouf, S. (2023). Revolutionizing the effect of Azadirachta indica extracts on edema induced changes in C-reactive protein and interleukin-6 in albino rats: in silico and in vivo approach. European Review for Medical & Pharmacological Sciences, 27(13), 5951-5963.

Aziz, T., Qadir, R., Anwar, F., Naz, S., Nazir, N., Nabi, G., Haiying, C., Lin, L., Alharbi, M., & Alasmari, A. F. (2024). Optimal Enzyme-Assisted Extraction of Phenolics from Leaves of Pongamia pinnata via Response Surface Methodology and Artificial Neural Networking. Applied Biochemistry and Biotechnology, 1-18. https://doi.org/10.1007/s12010-024-04875-w DOI: https://doi.org/10.1007/s12010-024-04875-w

Bawari, S., Sah, A. N., & Tewari, D. (2018). Antiurolithiatic activity of Daucus carota: an in vitro study. Pharmacognosy Journal, 10(5), 880-884. http://dx.doi.org/10.5530/pj.2018.5.148 DOI: https://doi.org/10.5530/pj.2018.5.148

Bhandari, R., Panta, A., Panth, P., Aryal, D., Gautam, U., Joshi, D. R., Kumar, R., Rokaya, T. B., Pandey, J., & Aryal, P. (2021). In-vitro comparison of anti-urolithiatic effect of Crataeva nurvala, Zea mays (corn silk) and ayurvedic formulation neeri tablet. International Journal of Pharmaceutical Sciences Review and Research, 66(1), 26-30. http://dx.doi.org/10.47583/ijpsrr.2021.v66i01.007 DOI: https://doi.org/10.47583/ijpsrr.2021.v66i01.007

Bharathi, B., Shivakumar, H., & Soumya, N. (2013). Depleted antioxidant vitamins and enhanced oxidative stress in urolithiasis. International Journal of Pharmacy and Biological Sciences, 3(1), 71-75.

Butterweck, V., & Khan, S. R. (2009). Herbal medicines in the management of urolithiasis: alternative or complementary? Planta Medica, 75(10), 1095-1103. https://doi.org/10.1055/s-0029-1185719 DOI: https://doi.org/10.1055/s-0029-1185719

Carović-Stanko, K., Liber, Z., Politeo, O., Strikić, F., Kolak, I., Milos, M., & Satovic, Z. (2011). Molecular and chemical characterization of the most widespread Ocimum species. Plant Systematics and Evolution, 294, 253-262. https://doi.org/10.1007/s00606-011-0471-x DOI: https://doi.org/10.1007/s00606-011-0471-x

Chander, P. A., Sri, H. Y., Sravanthi, N. B., & Susmitha, U. V. (2014). In vitro anthelmintic activity of Barleria buxifolia on Indian adult earthworms and estimation of total flavonoid content. Asian Pacific Journal of Tropical Disease, 4, S233-S235. https://doi.org/10.1016/S2222-1808(14)60445-X DOI: https://doi.org/10.1016/S2222-1808(14)60445-X

Devkar, R. A., Chaudhary, S., Adepu, S., Xavier, S. K., Chandrashekar, K. S., & Setty, M. M. (2016). Evaluation of antiurolithiatic and antioxidant potential of Lepidagathis prostrata: A Pashanbhed plant. Pharmaceutical Biology, 54(7), 1237-1245. https://doi.org/10.3109/13880209.2015.1066397 DOI: https://doi.org/10.3109/13880209.2015.1066397

Geetha, K., Manavalan, R., & Venkappaya, D. (2010). Effects and causes of Lithiasis. Research Journal of Pharmacology and Pharmacodynamics, 2(4), 261-267.

Hannan, J., Marenah, L., Ali, L., Rokeya, B., Flatt, P., & Abdel-Wahab, Y. (2006). Ocimum sanctum leaf extracts stimulate insulin secretion from perfused pancreas, isolated islets and clonal pancreatic beta-cells. Journal of Endocrinology, 189(1), 127-136. https://doi.org/10.1677/joe.1.06615 DOI: https://doi.org/10.1677/joe.1.06615

Hayat, P., Khan, I., Rehman, A., Jamil, T., Hayat, A., Rehman, M. U., Ullah, N., Sarwar, A., Alharbi, A. A., & Dablool, A. S. (2023). Myogenesis and analysis of antimicrobial potential of silver nanoparticles (AgNPs) against pathogenic bacteria. Molecules, 28(2), 637. https://doi.org/10.3390/molecules28020637 DOI: https://doi.org/10.3390/molecules28020637

Hong, S. Y., & Qin, B. L. (2023). The protective role of dietary polyphenols in urolithiasis: insights into antioxidant effects and mechanisms of action. Nutrients, 15(17), 3753. https://doi.org/10.3390/nu15173753 DOI: https://doi.org/10.3390/nu15173753

Hussain, A., Khan, A. A., Ali, M., Zamani, G. Y., Iqbal, Z., Ullah, Q., Iqbal, J., Shahzad, M., & Aziz, T. (2022). In-vitro and In-vivo assessment of toxic effects of Parthenium hysterophorus leaves extract. Journal of the Chilean Chemical Society, 67(2), 5484-5489. http://dx.doi.org/10.4067/S0717-97072022000205484 DOI: https://doi.org/10.4067/S0717-97072022000205484

Hussain, S., Nazmeen, N., & Aliuddin, S. (2019). Traditional Approach for the Management of Urolithiasis. International Journal of Pharmaceutical Sciences Review and Research, 54(1), 23-28.

Hussain, Z., Raza, M. A., Jahangeer, M., Sarwar, A., Nadeem, A. A., Naz, S., Aziz, T., Alharbi, M., Alshammari, A., & Alasmari, A. F. (2023). Green synthesis of silver nanoparticles prepared by leaves extract of Trigonila foenum-graecum and its antibacterial potential against Escherichia coli and Pseudomonas aeruginosa. Biomass Conversion and Biorefinery, 1-8. https://doi.org/10.1007/s13399-023-04852-z DOI: https://doi.org/10.1007/s13399-023-04852-z

Iqbal, J., Khan, A. A., Aziz, T., Ali, W., Ahmad, S., Rahman, S. U., Iqbal, Z., Dablool, A. S., Alruways, M. W., & Almalki, A. A. (2022). Phytochemical investigation, antioxidant properties and in vivo evaluation of the toxic effects of Parthenium hysterophorus. Molecules, 27(13), 4189. https://doi.org/10.3390/molecules27134189 DOI: https://doi.org/10.3390/molecules27134189

Kalyan, P., Kumar, M., Kavitha, K., Singh, J., & Khan, R. (2012). Pharmacological Actions of Ocimum sacntum–Review. International Journal of Advances in Pharmacy, Biology and Chemistry, 1(3), 406–414.

Kant, R., Singh, T. G., & Singh, S. (2020). Mechanistic approach to herbal formulations used for urolithiasis treatment. Obesity Medicine, 19, 100266. https://doi.org/10.1016/j.obmed.2020.100266 DOI: https://doi.org/10.1016/j.obmed.2020.100266

Khandelwal, K. (2008). Preliminary phytochemical screening (Chapter 40). In K. Khandelwal (Ed.), Practical Pharmacognosy-Techniques and Experiments: Nirali Prakashan.

Klein, R. (1996). Toxicology and herbs: an introduction. Australian Journal of Medical Herbalism, 8(4), 100–110. https://doi.org/10.3316/informit.409540743088133

Monisa, R., Azad, A., Rahma, I., Israt, J., Shopnil, A., Ayaz, A., Taqweem, U., Aziz, T., Metab, A., & Thamer, H. (2023). Assessing the hypolipidemic and gastro-liver protective activity of herbal combination with emphasis on PPI amid selected multiple antihypertensive drug combination in experimental animal models. European Review for Medical & Pharmacological Sciences, 27(22), 11021-11030.

Nagarajan, Y., Boopathi, R., Yahoob, S. A. M., & Venkatraman, A. (2019). In vitro evaluation of anti urolithiatic activity of Bryophyllum pinnatum Lam. In Vitro, 5(8), 97-102.

Naveed, M., Batool, H., Rehman, S. U., Javed, A., Makhdoom, S. I., Aziz, T., Mohamed, A. A., Sameeh, M. Y., Alruways, M. W., & Dablool, A. S. (2022a). Characterization and evaluation of the antioxidant, antidiabetic, anti-inflammatory, and cytotoxic activities of silver nanoparticles synthesized using Brachychiton populneus leaf extract. Processes, 10(8), 1521. https://doi.org/10.3390/pr10081521 DOI: https://doi.org/10.3390/pr10081521

Naveed, M., Bukhari, B., Aziz, T., Zaib, S., Mansoor, M. A., Khan, A. A., Shahzad, M., Dablool, A. S., Alruways, M. W., & Almalki, A. A. (2022b). Green synthesis of silver nanoparticles using the plant extract of Acer oblongifolium and study of its antibacterial and antiproliferative activity via mathematical approaches. Molecules, 27(13), 4226. https://doi.org/10.3390/molecules27134226 DOI: https://doi.org/10.3390/molecules27134226

Naveed, M., Makhdoom, S. I., Rehman, S. U., Aziz, T., Bashir, F., Ali, U., Alharbi, M., Alshammari, A., & Alasmari, A. F. (2023). Biosynthesis and mathematical interpretation of zero-valent iron NPs using Nigella sativa seed tincture for indemnification of carcinogenic metals present in industrial effluents. Molecules, 28(8), 3299. https://doi.org/10.3390/molecules28083299 DOI: https://doi.org/10.3390/molecules28083299

Nimavat, A., Trivedi, A., Yadav, A., & Patel, P. (2022). A Review on kidney stone and its herbal treatment. Journal of Pharmacy and Pharmacology, 10, 195-209. https://doi.org/10.17265/2328-2150/2022.06.003 DOI: https://doi.org/10.17265/2328-2150/2022.06.003

Ondziel-Opara, S. A., Atipo, A. M. O., Okemba, G. O., Mouss, R. B. B., Nyanga, Y. I. D., Odzebe, A. W. S., & Bouya, A. P. (2022). Epidemiological profile of patients suffering from urolithiasis in African Urological Environments from 2016 to 2020. Open Journal of Urology, 12(2), 157-167. https://doi.org/10.4236/oju.2022.122016 DOI: https://doi.org/10.4236/oju.2022.122016

Rahim, G., Qureshi, R., Hazrat, A., Ahmad, B., Ali Khan, A., Aziz, T., Alharbi, M., & Alshammari, A. (2023). Phytochemical, antimicrobial, radical scavenging and in-vitro biological activities of Teucrium stocksianum leaves. Journal of the Chilean Chemical Society, 68(1), 5748-5754. http://dx.doi.org/10.4067/S0717-97072023000105748 DOI: https://doi.org/10.4067/S0717-97072023000105748

Riaz, M., Nawaz, M., Qadir, R., Hussain, S., Roheen, T., Afzal, M., Perviaz, M., Ali, A., Aziz, T., & Alharbi, M. (2023). Characterization and antioxidant potential of white mustard (Brassica hirta) leaf extract and stabilization of sunflower oil. Open Chemistry, 21(1), 20230175. https://doi.org/10.1515/chem-2023-0175 DOI: https://doi.org/10.1515/chem-2023-0175

Saha, S., & Verma, R. J. (2013). Inhibition of calcium oxalate crystallisation in vitro by an extract of Bergenia ciliata. Arab Journal of Urology, 11(2), 187-192. https://doi.org/10.1016/j.aju.2013.04.001 DOI: https://doi.org/10.1016/j.aju.2013.04.001

Saleem, A., Afzal, M., Naveed, M., Makhdoom, S. I., Mazhar, M., Aziz, T., Khan, A. A., Kamal, Z., Shahzad, M., & Alharbi, M. (2022). HPLC, FTIR and GC-MS analyses of Thymus vulgaris phytochemicals executing in vitro and in vivo biological activities and effects on COX-1, COX-2 and gastric cancer genes computationally. Molecules, 27(23), 8512. https://doi.org/10.3390/molecules27238512 DOI: https://doi.org/10.3390/molecules27238512

Sathish, R., & Jayebalan, G. (2017). Study of in vitro anti-lithiatic effect of Ipomoea batatas (L) leaves and tuberous roots. Asian Journal of Pharmaceutical and Clinical Research, 11(2), 427-431. DOI: https://doi.org/10.22159/ajpcr.2018.v11i2.23319

Shabbir, M. A., Naveed, M., Rehman, S. U., Ain, N. U., Aziz, T., Alharbi, M., Alsahammari, A., & Alasmari, A. F. (2023). Synthesis of iron oxide nanoparticles from Madhuca indica plant extract and assessment of their cytotoxic, antioxidant, anti-inflammatory, and anti-diabetic properties via different nanoinformatics approaches. ACS Omega, 8(37), 33358-33366. https://doi.org/10.1021/acsomega.3c02744 DOI: https://doi.org/10.1021/acsomega.3c02744

Shah, S. W. A., Siddique Afridi, M., Ur-Rehman, M., Hayat, A., Sarwar, A., Aziz, T., Alharbi, M., Alshammari, A., & Alasmari, A. F. (2023). In vitro evaluation of phytochemicals, heavy metals and antimicrobial activities of leaf, stem and roots extracts of Caltha palustris var. alba. Journal of the Chilean Chemical Society, 68(1), 5807-5812. http://dx.doi.org/10.4067/S0717-97072023000105807 DOI: https://doi.org/10.4067/S0717-97072023000105807

Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144-158. https://doi.org/10.5344/ajev.1965.16.3.144 DOI: https://doi.org/10.5344/ajev.1965.16.3.144

Smyslova, O. A., Markaryan, A. A., Evdokimova, O. V., Glazkova, I. U., & Yaroshenko, M. A. (2015). Characteristics of the new comprehensive herbal medicine for the treatment and prevention of urolithiasis. Biology and Medicine, 7(2).

Soni, N., Gill, D., Sagar, B. S., Raheja, S., & Agrawal, S. (2012). Ocimum kilimandscharicum: A systematic review. Journal of Drug Delivery and Therapeutics, 2(3), 45-52. https://doi.org/10.22270/jddt.v2i3.169 DOI: https://doi.org/10.22270/jddt.v2i3.169

Waseem, M., Naveed, M., Rehman, S. U., Makhdoom, S. I., Aziz, T., Alharbi, M., Alsahammari, A., & Alasmari, A. F. (2023). Molecular characterization of spa, hld, fmhA, and l ukD genes and computational modeling the multidrug resistance of Staphylococcus species through Callindra harrisii silver nanoparticles. ACS Omega, 8(23), 20920-20936. https://doi.org/10.1021/acsomega.3c01597 DOI: https://doi.org/10.1021/acsomega.3c01597

Zahid, H., Shahab, M., Ur Rahman, S., Iqbal, Z., Khan, A., Aziz, T., Ali, W., Zamani, G., Ahmad, S., & Shahzad, M. (2022). Assessing the effect of walnut (Juglans regia) and olive (Olea europaea) oil against the bacterial strains found in Gut Microbiome. Progress in Nutrition, 24(3), e2022122. https://doi.org/10.23751/pn.v24i3.13311

Zahra, N., Fareed, T., Hamza, M., Zeshan, B., Sarwar, A., Aziz, T., Alharbi, M., Alshammari, A., & Alasmari, A. F. (2023). In-vivo and in-silico analysis of anti-inflammatory, analgesic, and anti pyretic activities of in-silico leaf extract. Journal of the Chilean Chemical Society, 68(2), 5813-5821. http://dx.doi.org/10.4067/s0717-97072023000205813 DOI: https://doi.org/10.4067/s0717-97072023000205813

Zaveri, M., Desai, N., & Movaliya, V. (2011). Effect of Ocimum basilicum on cisplatin models of acute renal failure. Advance Research in Pharmaceuticals and Biologicals, 1(2), 91-100.