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International Journal of Plant Based Pharmaceuticals
e‑ISSN 2791-7509 Open Access · CC BY 4.0
Vol. 4 · Issue 2 · 2024 Nov 30, 2024 Research Articles

Ameliorative effect of zingerone on cadmium-induced nephrotoxicity in adult wistar rats

AO
Augustine Oviosun Kampala International University, Faculty of Biomedical Sciences, Department of Anatomy, Western Campus, Ishaka-Bushenyi, Uganda Uganda
EA
Emeka Godson Anyanwu University of Nigeria, Faculty of Basic Medical Sciences, Department of Anatomy, Enugu Campus, Enugu State, Nigeria Nigeria
EO
Ezinne Chidinma Oviosun Corresponding Ambrose Alli University, Faculty of Basic Medical Sciences, Department of Anatomy, Ekpoma, Edo State, Nigeria oviosunec@gmail.com Nigeria
OB
Okwara Blasius Okechukwu University of Nigeria Teaching Hospital, Department of Orthopaedic and Trauma, Ituku/Ozalla, Enugu, Nigeria Nigeria
Pages118-124 PublishedNov 30, 2024 LicenseOpen Access
Full Text
IJPBP 2 VOL 4 · 2
VOL 4 · NO 2 · 2024 View issue

Abstract

Exposure to heavy metals like cadmium has been reported to cause severe kidney damage through oxidative stress and inflammation. Zingerone is a bioactive compound present in ginger, it contains significant anti-oxidative and anti-inflammatory properties. This study aims to investigate the anti-oxidative and therapeutic role of zingerone on cadmium-induced nephrotoxicity. Thirty (30) adult male rats were divided into 6 groups (A-F) of 5 rats each ( n = 5) randomly [A: normal control (normal saline), B: cadmium-exposed (5 mg/kg of cadmium only), C: zingerone-alone, D-F: 5 mg/kg of cadmium + 50 mg/kg, 100mg/kg, 200 mg/kg of zingerone, respectively]. Nephrotoxicity was induced by oral administration of cadmium chloride (CdCl₂), followed by zingerone treatment orally. Renal function markers (serum creatinine and urea level), oxidative stress markers (superoxide dismutases, catalase, malondialdehyde), and histopathological investigations of the kidney were assessed to evaluate the effects. Cadmium administration resulted in significant renal dysfunction, characterized by elevated serum creatinine, urea, and kidney malondialdehyde levels, along with reduced antioxidant enzyme activities (superoxide dismutase and catalase). Histopathological evaluation showed extensive kidney damage characterized by renal tubular damage, necrosis, and inflammation. Zingerone treatment significantly ameliorated these alterations, restoring renal function markers, reducing oxidative stress, and improving the histological architecture of the kidney. These findings suggest that zingerone exerts an anti-oxidative and therapeutic effect against cadmium-induced nephrotoxicity. According to these findings, zingerone shows potential as a therapeutic approach for kidney impairment caused by exposure to heavy metals.

Keywords

#Zingerone #Oxidative stress #Renal toxicity #Cadmium #Creatinine #Urea

References

  1. Aebi, H. (1984). Catalase in vitro (Vol. 105): Elsevier. https://doi.org/10.1016/S0076-6879(84)05016-3 DOI: https://doi.org/10.1016/S0076-6879(84)05016-3
  2. Ahmad, B., Rehman, M. U., Amin, I., Arif, A., Rasool, S., Bhat, S. A., Afzal, I., Hussain, I., Bilal, S., & Mir, M. U. R. (2015). A review on pharmacological properties of zingerone (4‐(4‐Hydroxy‐3‐methoxyphenyl)‐2‐butanone). The Scientific World Journal, 2015, 816364. http://dx.doi.org/10.1155/2015/816364 DOI: https://doi.org/10.1155/2015/816364
  3. Akinyemi, A. J., Faboya, O. L., Paul, A. A., Olayide, I., Faboya, O. A., & Oluwasola, T. A. (2018). Nephroprotective effect of essential oils from ginger (Zingiber officinale) and turmeric (Curcuma longa) rhizomes against cadmium-induced nephrotoxicity in rats. Journal of Oleo Science, 67(10), 1339-1345. https://doi.org/10.1155/2018/4109491 DOI: https://doi.org/10.5650/jos.ess18115
  4. Al-Naimi, M. S., Rasheed, H. A., Hussien, N. R., Al-Kuraishy, H. M., & Al-Gareeb, A. I. (2019). Nephrotoxicity: Role and significance of renal biomarkers in the early detection of acute renal injury. Journal of Advanced Pharmaceutical Technology & Research, 10(3), 95-99. https://doi.org/10.4103/japtr.JAPTR_336_18 DOI: https://doi.org/10.4103/japtr.JAPTR_336_18
  5. Alam, M. F. (2018). Neuroprotective effects of zingerone against carbon tetrachloride (CCl4) induced brain mitochondrial toxicity in Swiss albino mice. Journal of Applied and Natural Science, 10(2), 548-552. https://doi.org/10.31018/jans.v10i2.1734 DOI: https://doi.org/10.31018/jans.v10i2.1734
  6. Alibakhshi, T., Khodayar, M. J., Khorsandi, L., Rashno, M., & Zeidooni, L. (2018). Protective effects of zingerone on oxidative stress and inflammation in cisplatin-induced rat nephrotoxicity. Biomedicine & Pharmacotherapy, 105, 225-232. https://doi.org/10.1016/j.biopha.2018.05.085 DOI: https://doi.org/10.1016/j.biopha.2018.05.085
  7. Amin, I., Hussain, I., Rehman, M. U., Mir, B. A., Ganaie, S. A., Ahmad, S. B., Mir, M. U. R., Shanaz, S., Muzamil, S., & Arafah, A. (2021). Zingerone prevents lead‐induced toxicity in liver and kidney tissues by regulating the oxidative damage in Wistar rats. Journal of Food Biochemistry, 45(3), e13241. https://doi.org/10.1111/jfbc.13241 DOI: https://doi.org/10.1111/jfbc.13241
  8. Anusuya, N., Durgadevi, P., Dhinek, A., & Mythily, S. (2013). Nephroprotective effect of ethanolic extract of garlic (Allium sativum L.) on cisplatin induced nephrotoxicity in male wistar rats. Asian Journal of Pharmaceutical and Clinical Research, 6(4), 97-100.
  9. Augustine, O., Emeka, A. G., Ezinne, O., Chidinma, U. O. O., Emmanuel, O. N., & Oluwatoyin, M. E. (2023). Nephroprotective Properties of Aqueous Extract of Ficus exasperata on Gentamicin-Induced Nephrotoxicity. Acta Scientific Anatomy, 2(4), 12-18.
  10. Bannister, J., & Calabrese, L. (1987). Assays for superoxide dismutase. In D. Glick (Ed.), Methods of Biochemical Analysis (pp. 279–312): John Wiley & Sons, New York, NY, USA. DOI: https://doi.org/10.1002/9780470110539.ch5
  11. Bernard, A. (2008). Cadmium & its adverse effects on human health. Indian Journal of Medical Research, 128(4), 557-564.
  12. Bernhoft, R. A. (2013). Cadmium toxicity and treatment. The Scientific World Journal, 2013, 394652. https://doi.org/10.1155/2013/394652 DOI: https://doi.org/10.1155/2013/394652
  13. Ceretta, L. B., Réus, G. Z., Abelaira, H. M., Ribeiro, K. F., Zappellini, G., Felisbino, F. F., Steckert, A. V., Dal-Pizzol, F., & Quevedo, J. (2012). Increased oxidative stress and imbalance in antioxidant enzymes in the brains of alloxan‐induced diabetic rats. Journal of Diabetes Research, 2012, 302682. https://doi.org/10.1155/2012/302682 DOI: https://doi.org/10.1155/2012/302682
  14. Chargui, A., Zekri, S., Jacquillet, G., Rubera, I., Ilie, M., Belaid, A., Duranton, C., Tauc, M., Hofman, P., & Poujeol, P. (2011). Cadmium-induced autophagy in rat kidney: an early biomarker of subtoxic exposure. Toxicological Sciences, 121(1), 31-42. https://doi.org/10.1093/toxsci/kfr031 DOI: https://doi.org/10.1093/toxsci/kfr031
  15. Chiş, I., Baltaru, D., Dumitrovici, A., Coseriu, A., Radu, B., Moldovan, R., & Mureşan, A. (2016). Quercetin ameliorate oxidative/nitrosative stress in the brain of rats exposed to intermittent hypobaric hypoxia. Revista Virtual de Quimica, 8(2), 369-383. https://doi.org/10.5935/1984-6835.20160027 DOI: https://doi.org/10.5935/1984-6835.20160027
  16. Conti, M., Morand, P., Levillain, P., & Lemonnier, A. (1991). Improved fluorometric determination of malonaldehyde. Clinical Chemistry, 37(7), 1273-1275. https://doi.org/10.1093/clinchem/37.7.1273 DOI: https://doi.org/10.1093/clinchem/37.7.1273
  17. Das, S. C., & Al-Naemi, H. A. (2019). Cadmium toxicity: oxidative stress, inflammation and tissue injury. Scientific Research Publishing, 7, 144-163. http://dx.doi.org/10.4236/odem.2019.74012 DOI: https://doi.org/10.4236/odem.2019.74012
  18. Dawood, S. M., Mumtaz, F., & Padiya, R. (2022). Zingerone alleviates cadmium-induced nephrotoxicity in rats via its antioxidant and anti-apoptotic properties. Revista de Ciências Farmacêuticas Básica e Aplicada, 43, e759. https://doi.org/10.4322/2179-443X.0759 DOI: https://doi.org/10.4322/2179-443X.0759
  19. Ekor, M. (2014). Nephrotoxicity and nephroprotective potential of African medicinal plants. In V. Kuete (Ed.), Toxicological Survey of African Medicinal Plants (pp. 357-393): Elsevier. https://doi.org/10.1016/B978-0-12-800018-2.00012-1 DOI: https://doi.org/10.1016/B978-0-12-800018-2.00012-1
  20. Emeka, A. G., Augustine, O., Chidinma, O. E., & Nto, N. J. (2023). Zingerone improves memory impairment in Wistar rats exposed to cadmium via modulation of redox imbalance. Journal of Krishna Institute of Medical Sciences, 12(1), 3-16.
  21. Fatima, G., Raza, A. M., Hadi, N., Nigam, N., & Mahdi, A. A. (2019). Cadmium in human diseases: It’s more than just a mere metal. Indian Journal of Clinical Biochemistry, 34(4), 371-378. https://doi.org/10.1007/s12291-019-00839-8 DOI: https://doi.org/10.1007/s12291-019-00839-8
  22. Finn, W. F., & Porter, G. A. (2003). Urinary biomarkers and nephrotoxicity. In M. de Broe, G. Porter, W. Bennett, & G. Verpooten (Eds.), Clinical Nephrotoxins: Renal Injury from Drugs and Chemicals (pp. 621-655): Springer, Dordrecht. https://doi.org/10.1007/1-4020-2586-6_33 DOI: https://doi.org/10.1007/1-4020-2586-6_33
  23. Genchi, G., Sinicropi, M. S., Lauria, G., Carocci, A., & Catalano, A. (2020). The effects of cadmium toxicity. International Journal of Environmental Research and Public Health, 17(11), 3782. https://doi.org/10.3390/ijerph17113782 DOI: https://doi.org/10.3390/ijerph17113782
  24. Hogervorst, J., Plusquin, M., Vangronsveld, J., Nawrot, T., Cuypers, A., Van Hecke, E., Roels, H. A., Carleer, R., & Staessen, J. A. (2007). House dust as possible route of environmental exposure to cadmium and lead in the adult general population. Environmental Research, 103(1), 30-37. https://doi.org/10.1016/j.envres.2006.05.009 DOI: https://doi.org/10.1016/j.envres.2006.05.009
  25. Hosseinzadeh, A., Goudarzi, M., Karimi, M. Y., Khorsandi, L., Mehrzadi, S., & Mombeini, M. A. (2020). Zingerone ameliorates gentamicin-induced nephrotoxicity in rats. Comparative Clinical Pathology, 29, 971-980. https://doi.org/10.1007/s00580-020-03129-5 DOI: https://doi.org/10.1007/s00580-020-03129-5
  26. Jamakala, O., & Rani, A. U. (2014). Mitigating role of zinc and iron against cadmium induced toxicity in liver and kidney of male albino rat: a study with reference to metallothionein quantification. International Journal of Pharmacy and Pharmaceutical Sciences, 6(9), 411-417.
  27. Kandemir, F. M., Yildirim, S., Caglayan, C., Kucukler, S., & Eser, G. (2019). Protective effects of zingerone on cisplatin-induced nephrotoxicity in female rats. Environmental Science and Pollution Research, 26, 22562-22574. https://doi.org/10.1007/s11356-019-05505-3 DOI: https://doi.org/10.1007/s11356-019-05505-3
  28. Kim, K. S., Lim, H. J., Lim, J. S., Son, J. Y., Lee, J., Lee, B. M., Chang, S. C., & Kim, H. S. (2018). Curcumin ameliorates cadmium-induced nephrotoxicity in Sprague-Dawley rats. Food and Chemical Toxicology, 114, 34-40. https://doi.org/10.1016/j.fct.2018.02.007 DOI: https://doi.org/10.1016/j.fct.2018.02.007
  29. Kumar, L., Harjai, K., & Chhibber, S. (2014). Recent update on multiple pharmacological benefits of zingerone: a quick review. American Journal of Phytomedicine and Clinical Therapeutics, 2, 693-704.
  30. Lee, Y. K., Park, E. Y., Kim, S., Son, J. Y., Kim, T. H., Kang, W. G., Jeong, T. C., Kim, K. B., Kwack, S. J., & Lee, J. (2014). Evaluation of cadmium-induced nephrotoxicity using urinary metabolomic profiles in sprague-dawley male rats. Journal of Toxicology and Environmental Health, Part A, 77(22-24), 1384-1398. https://doi.org/10.1080/15287394.2014.951755 DOI: https://doi.org/10.1080/15287394.2014.951755
  31. Mani, V., Siddique, A. I., Arivalagan, S., Thomas, N. S., & Namasivayam, N. (2016). Zingerone ameliorates hepatic and renal damage in alcohol-induced toxicity in experimental rats. International Journal of Nutrition, Pharmacology, Neurological Diseases, 6(3), 125-132. https://doi.org/10.4103/2231-0738.184585 DOI: https://doi.org/10.4103/2231-0738.184585
  32. Mashhadi, N. S., Ghiasvand, R., Askari, G., Hariri, M., Darvishi, L., & Mofid, M. R. (2013). Anti-oxidative and anti-inflammatory effects of ginger in health and physical activity: review of current evidence. International Journal of Preventive Medicine, 4(Suppl 1), S36-S42.
  33. Matović, V., Buha, A., Ðukić-Ćosić, D., & Bulat, Z. (2015). Insight into the oxidative stress induced by lead and/or cadmium in blood, liver and kidneys. Food and Chemical Toxicology, 78, 130-140. https://doi.org/10.1016/j.fct.2015.02.011 DOI: https://doi.org/10.1016/j.fct.2015.02.011
  34. Mehrzadi, S., Khalili, H., Fatemi, I., Malayeri, A., Siahpoosh, A., & Goudarzi, M. (2021). Zingerone mitigates carrageenan-induced inflammation through antioxidant and anti-inflammatory activities. Inflammation, 44, 186-193. https://doi.org/10.1007/s10753-020-01320-y DOI: https://doi.org/10.1007/s10753-020-01320-y
  35. Munisamy, R., Ismail, S. N. S., & Praveena, S. M. (2013). Cadmium exposure via food crops: a case study of intensive farming area. American Journal of Applied Sciences, 10(10), 1252-1262. https://doi.org/10.3844/ajassp.2013.1252.1262 DOI: https://doi.org/10.3844/ajassp.2013.1252.1262
  36. Olubunmi, O. P., Yinka, O. S., Oladele, O. J., John, O. A., & Boluwatife, B. D. (2017). Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of Phyllanthus amarus in Cadmium Induced Kidney Damage in Experimental Animals. International Journal of Clinical and Developmental Anatomy, 3(4), 25-35. https://doi.org/10.11648/j.ijcda.20170304.12 DOI: https://doi.org/10.11648/j.ijcda.20170304.12
  37. Onwuka, F. C., Erhabor, O., Eteng, M., & Umoh, I. (2011). Protective effects of ginger toward cadmium-induced testes and kidney lipid peroxidation and hematological impairment in albino rats. Journal of Medicinal Food, 14(7-8), 817-821. https://doi.org/10.1089/jmf.2010.0106 DOI: https://doi.org/10.1089/jmf.2010.0106
  38. Oviosun, A., Emeka, A. G., Chidinma, O. E., Emmanuel, O. N., Ehizokhale, E. S., & Anthony, E. I. (2023). Therapeutic Properties of Ficus exasperata Extract on Gentamicin Induced Kidney Damage. European Journal of Pharmaceutical and Medical Research, 10(10), 50-55.
  39. Peana, M., Pelucelli, A., Chasapis, C. T., Perlepes, S. P., Bekiari, V., Medici, S., & Zoroddu, M. A. (2022). Biological effects of human exposure to environmental cadmium. Biomolecules, 13(1), 36. https://doi.org/10.3390/biom13010036 DOI: https://doi.org/10.3390/biom13010036
  40. Safhi, M. M. (2015). Zingerone protects the tellurium toxicity in the brain mitochondria of rats. Metabolomics, 5(4), 156. DOI: https://doi.org/10.4172/2153-0769.1000156
  41. Salama, M. E., Adel, M., Helal, G., & El-Shafey, M. (2019). Role of oxidative stress, apoptosis and autophagy in cadmium-induced renal injury in rats: renoprotective effect of ghrelin. Bulletin of Egyptian Society for Physiological Sciences, 39(2), 271-285. https://doi.org/10.1016/j.lfs.2021.120121 DOI: https://doi.org/10.21608/besps.2019.14414.1025
  42. Sevastre-Berghian, A. C., Făgărăsan, V., Toma, V. A., Bâldea, I., Olteanu, D., Moldovan, R., Decea, N., Filip, G. A., & Clichici, S. V. (2017). Curcumin reverses the diazepam‐induced cognitive impairment by modulation of oxidative stress and ERK 1/2/NF‐κB pathway in brain. Oxidative Medicine and Cellular Longevity, 2017(1), 3037876. https://doi.org/10.1155/2017/3037876 DOI: https://doi.org/10.1155/2017/3037876
  43. Shati, A. A. (2011). Effects of Origanum majorana L. on cadmium induced hepatotoxicity and nephrotoxicity in albino rats. Saudi Medical Journal, 32(8), 797-805.
  44. Spencer, L., Bancroft, J., Bancroft, J., & Gamble, M. (2012). Tissue processing: (pp. 105–123). Netherlands, Amsterdam: Elsevier Health Sciences. DOI: https://doi.org/10.1016/B978-0-7020-4226-3.00006-8
  45. Stevens, L. A., Coresh, J., Greene, T., & Levey, A. S. (2006). Assessing kidney function—measured and estimated glomerular filtration rate. New England Journal of Medicine, 354(23), 2473-2483. https://doi.org/10.1056/NEJMra054415 DOI: https://doi.org/10.1056/NEJMra054415
  46. Türk, E., Güvenç, M., Cellat, M., Uyar, A., Kuzu, M., Ağgül, A. G., & Kırbaş, A. (2022). Zingerone protects liver and kidney tissues by preventing oxidative stress, inflammation, and apoptosis in methotrexate-treated rats. Drug and Chemical Toxicology, 45(3), 1054-1065. https://doi.org/10.1080/01480545.2020.1804397 DOI: https://doi.org/10.1080/01480545.2020.1804397
  47. Vijaya, P., Kaur, H., Garg, N., & Sharma, S. (2020). Protective and therapeutic effects of garlic and tomato on cadmium-induced neuropathology in mice. The Journal of Basic and Applied Zoology, 81, 23. https://doi.org/10.1186/s41936-020-00160-4 DOI: https://doi.org/10.1186/s41936-020-00160-4
  48. Vukićević, T. (2012). Toxic effects of cadmium. Acta Medica Mediterranea, 51(4), 65-70. https://doi.org/10.5633/amm.2012.0410
  49. Wachira, F. N., Areba, G., Ngure, R., Khalid, R., Maloba, F., Nyaga, N., Moseti, K., Ngotho, M., Wanyoko, J., & Karori, S. (2019). Neuroprotective effects of tea against cadmium toxicity. Bioactive Compounds in Health and Disease, 2(12), 230-246. DOI: https://doi.org/10.31989/bchd.v2i12.684
  50. Yan, L. J., & Allen, D. C. (2021). Cadmium-induced kidney injury: Oxidative damage as a unifying mechanism. Biomolecules, 11(11), 1575. https://doi.org/10.3390/biom11111575 DOI: https://doi.org/10.3390/biom11111575
  51. Zhang, Y. X. (2012). Simultaneous determination of five gingerols in raw and processed ginger by HPLC. Chinese Pharmaceutical Journal, 24, 471-474.

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