Synthesis, characterization, antibacterial, antioxidant activity, and lipoxygenase enzyme inhibition profile of silver nanoparticles (AgNPs) by green synthesis from Seseli resinosum Freyn & Sint

Abstract

In the study, silver nanoparticles (AgNPs) were successfully synthesized by an environmentally friendly synthesis method using Seseli resinosum Freyn & Sint extract. The synthesized silver nanoparticles were characterized by ultraviolet/visible light absorption spectrophotometer (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analysis. As a result of the characterization, it was determined that 33 nm spherical nanoparticles were formed, showing a spectrum at ~420 nm wavelength. Silver nanoparticles showed a bacteriocidal effect against all bacterial strains. DPPH and ABTS methods were used to examine the antioxidant activities of plant extracts and AgNPs. In DPPH removal activity, AgNPs obtained by green synthesis provided a high rate of inhibition removal compared to the extract. According to this percentage, while silver nanoparticles provided 22% removal, the extract provided 15% removal. In ABTS removal activity, when AgNPs were obtained by green synthesis compared to the extract, silver nanoparticles provided 25% removal, while the extract provided 18% removal. The characterization of silver nanoparticles synthesized by the green synthesis method and their antioxidant activity were investigated, and the obtained values indicate the presence of an antioxidant capacity. In addition, the inhibitory effects of the extract and AgNP on lipoxygenase activity, which has an important place in health, were investigated. It was determined that the aqueous extract of S. resinosum and the AgNP synthesized from the extract had lipoxygenase enzyme inhibitory activity.

Keywords

References

1. Agnihotri, S., Mukherji, S., Mukherji, S., 2014. Size-controlled silver nanoparticles synthesized over the range 5-100 nm using the same protocol and their antibacterial efficacy. RSC Advances, 4(8), 3974-3983.
2. Ahmed, S., Ahmad, M., Swami, B.L., Ikram, S., 2016. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. Journal of Advanced Research, 7(1), 17-28.
3. Almeida, A.D.R., Maciel, M.V.D.O.B., Machado, M.H., Bazzo, G.C., de Armas, R.D., Vitorino, V.B., Barreto, P.L.M., 2020. Bioactive compounds and antioxidant activities of Brazilian hop (Humulus lupulus L.) extracts. International Journal of Food Science & Technology, 55(1), 340-347.
4. Al-Ogaidi, I., Salman, M.I., Mohammad, F.I., Aguilar, Z., Al-Ogaidi, M., Hadi, Y.A., Abd Al-Rhman, R.M., 2017. Antibacterial and cytotoxicity of silver nanoparticles synthesized in green and black tea. World Journal of Experimental Biosciences, 5(1), 39-45.
5. Alsammarraie, F.K., Wang, W., Zhou, P., Mustapha, A., Lin, M., 2018. Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities. Colloids and Surfaces B: Biointerfaces, 171, 398-405.
6. Anthon, G.E., Barrett, D.M., 2012. Pectin methylesterase activity and other factors affecting pH and titratable acidity in processing tomatoes. Food Chemistry, 132(2), 915-920.
7. Antony, J.J., Nivedheetha, M., Siva, D., Pradeepha, G., Kokilavani, P., Kalaiselvi, S., Achiraman, S., 2013. Antimicrobial activity of Leucas aspera engineered silver nanoparticles against Aeromonas hydrophila in infected Catla catla. Colloids and Surfaces B: Biointerfaces, 109, 20-24.
8. Banala, R.R., Nagati, V.B., Karnati, P.R., 2015. Green synthesis and characterization of Carica papaya leaf extract coated silver nanoparticles through X-ray diffraction, electron microscopy and evaluation of bactericidal properties, Saudi Journal of Biological Sciences, 22(5), 637-644.
9. Chen, J.C., Lin, Z.H., Ma, X.X., 2003. Evidence of the production of silver nanoparticles via pretreatment of Phoma sp. 3.2883 with silver nitrate. Letters in Applied Microbiology, 37(2), 105-108.
10. Davis, P.H., 1970. Flora of Turkey and the East Aegean Islands. Vol. 3. Flora of Turkey and the East Aegean Islands. Vol. 3.
11. Dipankar, C., Murugan, S., 2012. The green synthesis, characterization and evaluation of the biological activities of silver nanoparticles synthesized from Iresine herbstii leaf aqueous extracts. Colloids and Surfaces B: Biointerfaces, 98, 112-119.
12. Eren, A., Baran, M.F., 2019. Green synthesis, characterization and antimicrobial activity of silver nanoparticles (AgNPs) from maize (Zea mays L.). Applied Ecology and Environmental Research, 17(2), 4097-4105.
13. Francis, S., Joseph, S., Koshy, E.P., Mathew, B., 2017. Green synthesis and characterization of gold and silver nanoparticles using Mussaenda glabrata leaf extract and their environmental applications to dye degradation. Environmental Science and Pollution Research, 24(21), 17347-17357.
14. Geethalakshmi, R., Sarada, D.V.L., 2010. Synthesis of plant-mediated silver nanoparticles using Trianthema decandra extract and evaluation of their anti microbial activities. International Journal of Engineering Science and Technology, 2(5), 970-975.
15. Genc, N., 2021. Biosynthesis of silver nanoparticles using Origanum onites extract and investigation of their antioxidant activity. Particulate Science and Technology, 39(5), 562-568.
16. Genc, N., Yildiz, I., Chaoui, R., Erenler, R., Temiz, C., Elmastas, M., 2020. Biosynthesis, characterization and antioxidant activity of oleuropein-mediated silver nanoparticles. Inorganic and Nano-Metal Chemistry, 51(3), 411-419.
17. Gopinath, K., Devi, N.P., Govindarajan, M., Bhakyaraj, K., Kumaraguru, S., Arumugam, A., Benelli, G., 2017. One-Pot green synthesis of silver nanoparticles using the orchid leaf extracts of Anoectochilus elatus: growth inhibition activity on seven microbial pathogens. Journal of Cluster Science, 28(3), 1541-1550.
18. Gopinath, K., Kumaraguru, S., Bhakyaraj, K., Mohan, S., Venkatesh, K.S., Esakkirajan, M., Arumugam, A., 2016. Green synthesis of silver, gold and silver/gold bimetallic nanoparticles using the Gloriosa superba leaf extract and their antibacterial and antibiofilm activities. Microbial Pathogenesis, 101, 1-11.
19. Guzel, A., Aksit, H., Elmastas, M., Erenler, R., 2017. Bioassay-guided isolation and identification of antioxidant flavonoids from Cyclotrichium origanifolium (Labill.) manden and scheng. Pharmacognosy Magazine, 13(50), 316-320.
20. Halliwell, B., 1990. How to characterize a biological antioxidant. Free Radical Research Communications, 9(1), 1-32.
21. Han, H., Yılmaz, H., Gulcin, I. 2018. Antioxidant activity of flaxseed (Linum usitatissimum L.) and analysis of its polyphenol contents by LC-MS/MS. Records of Natural Products, 12(4),397–402.
22. Hudzicki, J., 2009. Kirby-Bauer disk diffusion susceptibility test protocol. American Society for Microbiology, 15, 55-63.
23. Juntachote, T., Berghofer, E., 2005. Antioxidative properties and stability of ethanolic extracts of Holy basil and Galangal. Food Chemistry, 92(2), 193-202.
24. Kelavkar, U.P., Cohen, C., Kamitani, H., Eling, T.E., Badr, K.F. 2000. Concordant induction of 15-lipoxygenase-1 and mutant p53 expression in human prostate adenocarcinoma: correlation with Gleason stating. Carcinogenesis, 21,1777-1787.
25. Kung, J.C., Chen, Y.J., Chiang, Y.C., Lee, C.L., Yang-Wang, Y.T., Hung, C.C., Shih, C.J., 2018. Antibacterial activity of silver nanoparticle (AgNP) confined mesoporous structured bioactive powder against Enterococcus faecalis infecting root canal systems. Journal of Non-Crystalline Solids, 502(15), 62-70.
26. Lončarić, M., Strelec, I., Moslavac, T., Šubarić, D., Pavić, V., Molnar, M., 2021. Lipoxygenase inhibition by plant extracts. Biomolecules, 11(2), 152.
27. Onar, H.C., Yusufoglu, A., Turker, G., Yanardag, R., 2012. Elastase, tyrosinase and lipoxygenase inhibition and antioxidant activity of an aqueous extract from Epilobium angustifolium L. leaves. Journal of Medicinal Plants Research, 6(5), 716-726.
28. Pallela, P.N.V.K., Ummey, S., Ruddaraju, L.K., Pammi, S.V.N., Yoon, S.G., 2018. Ultra Small, mono dispersed green synthesized silver nanoparticles using aqueous extract of Sida cordifolia plant and investigation of antibacterial activity. Microbial Pathogenesis, 124, 63-69.
29. Premkumar, J., Sudhakar, T., Dhakal, A., Shrestha, J.B., Krishnakumar, S., Balashanmugam, P., 2018. Synthesis of silver nanoparticles (AgNPs) from cinnamon against bacterial pathogens. Biocatalysis and Agricultural Biotechnology, 15, 311-316.
30. Rather, M.A., Sharma, R., Gupta, S., Ferosekhan, S., Ramya, V.L., Jadhao, S.B., 2013. Chitosan-nanoconjugated hormone nanoparticles for sustained surge of gonadotropins and enhanced reproductive output in female fish. PloS one, 8(2), e57094.
31. Samuelsson, B., Dahlen, S.E., Lindgren, J.Å., Rouzer, C.A., Serhan, C.N., 1987. Leukotrienes and lipoxins: structures, biosynthesis, and biological effects. Science, 237(4819), 1171-1176.
32. Saravanakumar, K., Chelliah, R., Shanmugam, S., Varukattu, N.B., Oh, D.H., Kathiresan, K., Wang, M.H., 2018. Green synthesis and characterization of biologically active nanosilver from seed extract of Gardenia jasminoides Ellis. Journal of Photochemistry and Photobiology B: Biology, 185, 126-135.
33. Selvakumar, P., Sithara, R., Viveka, K., Sivashanmugam, P., 2018. Green synthesis of silver nanoparticles using leaf extract of Acalypha hispida and its application in blood compatibility. Journal of Photochemistry and Photobiology B: Biology, 182, 52-61.
34. Sindhi ,V., Gupta, V., Sharma, K., Bhatnagar, S., Kumari, R., Dhaka, N. 2013. Potential applications of antioxidants—a review. Journal of Pharmacy Research, 7,828–835.
35. Singhal, G., Bhavesh, R., Kasariya, K., Sharma, A.R., Singh, R.P., 2011. Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. Journal of Nanoparticle Research, 13(7), 2981-2988.
36. Wang, M., Zhang, W., Zheng, X., Zhu, P., 2017. Antibacterial and catalytic activities of biosynthesized silver nanoparticles prepared by using an aqueous extract of green coffee bean as a reducing agent. RSC Advances, 7(20), 12144-12149.
37. Xu, H., Shi, X., Ma, H., Lv, Y., Zhang, L., Mao, Z., 2011. The preparation and antibacterial effects of dopa-cotton/AgNPs. Applied Surface Science, 257(15), 6799-6803.
38. Yang, G., Xie, J., Deng, Y., Bian, Y., Hong, F., 2012. Hydrothermal synthesis of bacterial cellulose/AgNPs composite: a “green” route for antibacterial application. Carbohydrate Polymers, 87(4), 2482-2487.
39. Zhang, X.F., Liu, Z.G., Shen, W.,Gurunathan, S., 2016. SilverNanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches. International Journal of Molecular Sciences, 17, 1534.

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