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International Journal of Plant Based Pharmaceuticals
e‑ISSN 2791-7509 Open Access · CC BY 4.0
Vol. 2 · Issue 2 · 2022 Sep 5, 2022 Research Articles

The antibiofilm effects of some Cistus spp. against pathogenic microorganisms

SE
Sevim Feyza Erdoğmuş Corresponding Afyonkarahisar Health Sciences University, Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Afyonkarahisar, Turkey feyzakus@gmail.com Turkey
CB
Cihat Bilecen Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Medical Biology and Genetics, Afyonkarahisar, Turkey Turkey
ÖE
Özlem Erdal Altıntaş Afyonkarahisar Health Sciences University, Şuhut Vocational School of Health Services, Department of Medical Services and Techniques, Afyonkarahisar, Turkey Turkey
SU
Sevgi Ulukütük Afyon Kocatepe University, Şuhut Vocational School, Department of Food Processing, Afyonkarahisar, Turkey Turkey
MK
Mustafa Kargıoğlu Afyon Kocatepe University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Turkey Turkey
Pages252-260 PublishedSep 5, 2022 LicenseOpen Access
Full Text
IJPBP 2 VOL 2 · 2
VOL 2 · NO 2 · 2022 View issue

Abstract

Recently, the potential antibacterial or antibiofilm effects of some plant species belonging to the Cistus sp. have motivated investigation of their use as herbal remedies. In this study, antibiofilm activities of aqueous (dH 2 O) leaf extracts of Cistus laurifolius L., C. creticus L. and C. salviifolius L. on some pathogenic microorganisms with biofilm forming ability were investigated. Biofilm forming ability of pathogen test microorganisms were evaluated by congo red agar method and microtiter plate method and all tested microorganisms were confirmed as biofilm producers. Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 11778, S. aureus ATCC 6538, P. aeruginosa ATCC 27853 and S. aureus ATCC 12600 were evaluated as strong biofilm producers. The highest concentration of examined extracts both showed biofilm inhibition and biofilm eradication against the tested pathogen microorganisms. In particular, studied plant extracts showed good antibiofilm effect, and biofilm eradication against S. aureus ATCC 6538 and S. aureus ATCC 12600. MBIC 50 values of S. aureus ATCC 6538 were found as 6.25 µg/ml of C. laurifolius , and 50 µg/ml of C. creticus extracts. Also, 50 µg/ml of C. creticus extract showed ≥ 90% inhibition of biofilm growth (MBIC 90 = 50 µg/ml). MBEC 50 values of S. aureus ATCC 12600 were determined as 6.25 µg/ml in all tested plant extracts and 50 µg/ml of C. creticus extract was required to induce ≥ 90% eradication (MBEC 90 ) of biofilm growth of S. aureus ATCC 12600. Our study revealed that aqueous leaf extracts of C. laurifolius , C. creticus and C. salviifolius could be potential candidates for drug discovery to treat pathogen test microorganisms capable to induce infectious diseases especially by their biofilm forming ability.

Keywords

#Antibiofilm #Cistus creticus L. # Cistus laurifolius L. #Cistus salviifolius L. #Pathogen microorganism

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Copyright (c) 2022 Sevim Feyza Erdoğmuş, Cihat Bilecen, Özlem Erdal Altıntaş, Sevgi Ulukütük, Mustafa Kargıoğlu

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