Comparative study on phenolic content, flavonoid content, and antioxidant activities of five species of the genus Phaseolus

Firdose R. Kolar; Vinutadivya Nirmanik; Annapurna Kagawad; Laxmi Angadi; Babu R. Lamani

doi:10.62313/ijpbp.2022.24

Authors

Firdose R. Kolar Karnataka State Akkamahadevi Women’s University, Department of Botany, Vijayapura-586 108, Karnataka, India https://orcid.org/0000-0001-7482-8802
Vinutadivya Nirmanik Karnataka State Akkamahadevi Women’s University, Department of Botany, Vijayapura-586 108, Karnataka, India https://orcid.org/0000-0002-5749-3219
Annapurna Kagawad Karnataka State Akkamahadevi Women’s University, Department of Botany, Vijayapura-586 108, Karnataka, India https://orcid.org/0000-0000-0000-0001
Laxmi Angadi Karnataka State Akkamahadevi Women’s University, Department of Botany, Vijayapura-586 108, Karnataka, India https://orcid.org/0000-0001-5798-0976
Babu R. Lamani Karnataka State Akkamahadevi Women’s University, Department of Botany, Vijayapura-586 108, Karnataka, India https://orcid.org/0000-0002-6716-8380

DOI:

https://doi.org/10.62313/ijpbp.2022.24

Keywords:

Fabaceae, Phaseolus, Phytochemicals, Antioxidants, Radical scavenging, Correlation

Abstract

The current study was designed to assess five species of the genus Phaseolus for phenolic content, flavonoid content, and antioxidant ability. The antioxidant capacity of the sample extracts was assessed using different antioxidant models such as ferric reducing antioxidant power (FRAP), DPPH free radical scavenging, phosphomolybdenum reducing power, ferrous ion chelating activity, hydrogen peroxide radical scavenging, hydroxyl radical scavenging, deoxyribose degradation, and β-carotene bleaching assays. The results obtained discovered that the concentration of phenolics and flavonoids in the studied species ranged from 1.11 to 4.01mg TAE/g plant material and 0.11 to 1.16 mg QE/g plant material. The antioxidant activity of the extracts varied in a wide range in the different antioxidant assays depending on the genotype as well as the polarity of the solvents used to obtain the extracts. Ethanolic and aqueous extracts exhibited the maximum amount of phenolics and flavonoids among the solvents. The species studied exhibited a significant range of phenolics, flavonoids, and antioxidant capacity. Hence, the present investigation can provide a new direction by utilizing Phaseolus species to formulate cost-effective, eco-friendly, and value-added therapeutic products.

References

Aknod, A.S.M.G.M., Khandaker, L., Berthold, J., Gates, L., Peters, K., Delong, H., Hossain, K., 2011. Anthocyanin, total polyphenols and antioxidant activity of common bean. American Journal of Food Technology, 6, 385–394. DOI: https://doi.org/10.3923/ajft.2011.385.394

Alcázar-Valle, M., Lugo-Cervantes, E., Mojica, L., Morales-Hernández, N., Reyes-Ramírez, H., Enríquez-Vara, J.N., García-Morales, S., 2020. Bioactive compounds, antioxidant activity, and antinutritional content of legumes: a comparison between four phaseolus species. Molecules, 25(15), 3528. DOI: https://doi.org/10.3390/molecules25153528

Amarowicz, R., Pegg, R.B., 2008. Legumes as a source of natural antioxidants. European Journal of Lipid Science and Technology, 110(10), 865-878. DOI: https://doi.org/10.1002/ejlt.200800114

Aquino, R., Morelli, S., Lauro, M.R., Abdo, S., Saija, A., Tomaino, A., 2001. Phenolic constituents and antioxidant activity of an extract of anthurium v ersicolor leaves. Journal of Natural Products, 64(8), 1019-1023. DOI: https://doi.org/10.1021/np0101245

Arfan, M., Khan, R., Rybarczyk, A., Amarowicz, R., 2012. Antioxidant activity of mulberry fruit extracts. International Journal of Molecular Sciences, 13(2), 2472-2480. DOI: https://doi.org/10.3390/ijms13022472

Bezuhla, O.N., Pozdniakov, V.V., Kobyzeva, L.N., Boguslavskiy, R.L., Antsyferova, O.V., 2018. Antioxidant activity of Phaseolus L. species seeds with different coat colour. Zemes Ukio Mokslai, 25(3), 117-124. DOI: https://doi.org/10.6001/zemesukiomokslai.v25i3.3794

Câmara, C.R., Urrea, C.A., Schlegel, V., 2013. Pinto beans (Phaseolus vulgaris L.) as a functional food: implications on human health. Agriculture, 3(1), 90-111. DOI: https://doi.org/10.3390/agriculture3010090

Capistrán-Carabarin, A., Aquino-Bolaños, E.N., García-Díaz, Y.D., Chávez-Servia, J.L., Vera-Guzmán, A.M., Carrillo-Rodríguez, J.C., 2019. Complementarity in Phenolic Compounds and the Antioxidant Activities of Phaseolus coccineus L. and P. vulgaris L. Landraces. Foods, 8(8), 295. DOI: https://doi.org/10.3390/foods8080295

Chávez-Mendoza, C., Sánchez, E., 2017. Bioactive compounds from Mexican varieties of the common bean (Phaseolus vulgaris): Implications for health. Molecules, 22(8), 1360. DOI: https://doi.org/10.3390/molecules22081360

Chen, P.X., Tang, Y., Marcone, M.F., Pauls, P.K., Zhang, B., Liu, R., Tsao, R., 2015. Characterization of free, conjugated and bound phenolics and lipophilic antioxidants in regular-and non-darkening cranberry beans (Phaseolus vulgaris L.). Food Chemistry, 185, 298-308. DOI: https://doi.org/10.1016/j.foodchem.2015.03.100

Choung, M.G., Choi, B.R., An, Y.N., Chu, Y.H., Cho, Y.S., 2003. Anthocyanin profile of Korean cultivated kidney bean (Phaseolus vulgaris L.). Journal of Agricultural and Food Chemistry, 51(24), 7040-7043. DOI: https://doi.org/10.1021/jf0304021

Chun, O.K., Kim, D.O., Lee, C.Y., 2003. Superoxide radical scavenging activity of the major polyphenols in fresh plums. Journal of Agricultural and Food Chemistry, 51(27), 8067-8072. DOI: https://doi.org/10.1021/jf034740d

Curran, J., 2012. The nutritional value and health benefits of pulses in relation to obesity, diabetes, heart disease and cancer. British Journal of Nutrition, 108(S1), S1-S2. DOI: https://doi.org/10.1017/S0007114512003534

De Mejia, E.G., Guzmán-Maldonado, S.H., Acosta-Gallegos, J.A., Reynoso-Camacho, R., Ramírez-Rodríguez, E., Pons-Hernández, J.L., González-Chavira, M.M., Castellanos, J. Z., Kelly, J.D., 2003. Effect of cultivar and growing location on the trypsin inhibitors, tannins, and lectins of common beans (Phaseolus vulgaris L.) grown in the semiarid highlands of Mexico. Journal of Agricultural and Food Chemistry, 51(20), 5962-5966. DOI: https://doi.org/10.1021/jf030046m

Dehpour, A.A., Ebrahimzadeh, M.A., Fazel, N.S., Mohammad, N.S., 2009. Antioxidant activity of the methanol extract of Ferula assafoetida and its essential oil composition. Grasas y Aceites, 60(4), 405-412. DOI: https://doi.org/10.3989/gya.010109

Dinis, T.C.P., Madeira, V.M.C., Almeida, M.L.M., 1994. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315(1), 161-169. DOI: https://doi.org/10.1006/abbi.1994.1485

Espinosa-Alonso, L.G., Lygin, A., Widholm, J.M., Valverde, M.E., Paredes-Lopez, O., 2006. Polyphenols in wild and weedy Mexican common beans (Phaseolus vulgaris L.). Journal of Agricultural and Food Chemistry, 54(12), 4436-4444. DOI: https://doi.org/10.1021/jf060185e

Guajardo-Flores, D., Serna-Saldívar, S.O., Gutiérrez-Uribe, J.A., 2013. Evaluation of the antioxidant and antiproliferative activities of extracted saponins and flavonols from germinated black beans (Phaseolus vulgaris L.). Food Chemistry, 141(2), 1497-1503. DOI: https://doi.org/10.1016/j.foodchem.2013.04.010

Gupta, K., Maurya, S., Agarwal, S., Kushwaha, A., Kumar, R., Pandey, A.K., Singh, M.P., 2016. Antioxidant assessment of extracts obtained through hot extraction process. Cellular and Molecular Biology, 62(3), 129.

Gyamfi, M.A., Yonamine, M., Aniya, Y., 1999. Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries. General Pharmacology: The Vascular System, 32(6), 661-667. DOI: https://doi.org/10.1016/S0306-3623(98)00238-9

Halliwell, B., Clement, M.V., Long, L.H., 2000. Hydrogen peroxide in the human body. FEBS Letters, 486(1), 10-13. DOI: https://doi.org/10.1016/S0014-5793(00)02197-9

Halliwell, B., Gutteridge, J.M., Aruoma, O.I., 1987. The deoxyribose method: a simple “test-tube” assay for determination of rate constants for reactions of hydroxyl radicals. Analytical Biochemistry, 165(1), 215-219. DOI: https://doi.org/10.1016/0003-2697(87)90222-3

Hayat, I., Ahmad, A., Masud, T., Ahmed, A., Bashir, S., 2014. Nutritional and health perspectives of beans (Phaseolus vulgaris L.): an overview. Critical Reviews in Food Science and Nutrition, 54(5), 580-592. DOI: https://doi.org/10.1080/10408398.2011.596639

Khan, R.A., Khan, M.R., Sahreen, S., Ahmed, M., 2012. Evaluation of phenolic contents and antioxidant activity of various solvent extracts of Sonchus asper (L.) Hill. Chemistry Central Journal, 6(1), 1-7. DOI: https://doi.org/10.1186/1752-153X-6-12

Kim, M.Y., Seguin, P., Ahn, J.K., Kim, J.J., Chun, S.C., Kim, E.H., Chung, I.M., 2008. Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. Journal of Agricultural and Food Chemistry, 56(16), 7265-7270. DOI: https://doi.org/10.1021/jf8008553

Krishnaswamy, K., 1996. Indian functional foods: Role in prevention of cancer. Nutrition Reviews, 54(11), S127-S131. DOI: https://doi.org/10.1111/j.1753-4887.1996.tb03832.x

Lee, C.Y., Nanah, C.N., Held, R.A., Clark, A.R., Huynh, U.G., Maraskine, M.C., Sharma, A., 2015. Effect of electron donating groups on polyphenol-based antioxidant dendrimers. Biochimie, 111, 125-134. DOI: https://doi.org/10.1016/j.biochi.2015.02.001

Luximon-Ramma, A., Bahorun, T., Soobrattee, M.A., Aruoma, O.I., 2002. Antioxidant activities of phenolic, proanthocyanidin, and flavonoid components in extracts of Cassia fistula. Journal of Agricultural and Food Chemistry, 50(18), 5042-5047. DOI: https://doi.org/10.1021/jf0201172

Miller, H.E., 1971. A simplified method for the evaluation of antioxidants. Journal of the American Oil Chemists' Society, 48(2), 91. DOI: https://doi.org/10.1007/BF02635693

Oliveira, I., Valentão, P., Lopes, R., Andrade, P.B., Bento, A., Pereira, J.A., 2009. Phytochemical characterization and radical scavenging activity of Portulaca oleraceae L. leaves and stems. Microchemical Journal, 92(2), 129-134. DOI: https://doi.org/10.1016/j.microc.2009.02.006

Onyilagha, J.C., Islam, S., 2009. Flavonoids and other polyphenols of the cultivated species of the genus Phaseolus. International Journal of Agriculture and Biology, 11, 231-234.

Pitura, K., Arntfield, S.D., 2019. Characteristics of flavonol glycosides in bean (Phaseolus vulgaris L.) seed coats. Food Chemistry, 272, 26-32. DOI: https://doi.org/10.1016/j.foodchem.2018.07.220

Prieto, P., Pineda, M., Aguilar, M., 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry, 269(2), 337-341. DOI: https://doi.org/10.1006/abio.1999.4019

Pulido, R., Bravo, L., Saura-Calixto, F., 2000. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of Agricultural and Food Chemistry, 48(8), 3396-3402. DOI: https://doi.org/10.1021/jf9913458

Reische, D.W., Lillard, D.A., Eitenmiller, R.R., 2008. Antioxidants. In: Akoh C.C., Min D.B., editors. Food Lipids Chemistry Nutrition, and Biotechnology. 3rd. CRC Press; New York, NY, USA, p. 409-433. DOI: https://doi.org/10.1201/9781420046649.ch15

Rocha-Guzman, N.E., Gallegos-Infante, J.A., 2007. Antioxidant activity in cotyledon of black and yellow common beans (Phaseolus vulgaris L.). Research Journal of Biological Sciences, 2(1), 112-117.

Rodríguez Madrera, R., Campa Negrillo, A., Suárez Valles, B., Ferreira Fernández, J.J., 2021. Phenolic content and antioxidant activity in seeds of common bean (Phaseolus vulgaris L.). Foods, 10(4), 864. DOI: https://doi.org/10.3390/foods10040864

Rodríguez Madrera, R.R., Negrillo, A.C., Valles, B.S., Fernández, J.J.F., 2020. Characterization of extractable phenolic profile of common bean seeds (Phaseolus vulgaris L.) in a Spanish diversity panel. Food Research International, 138, 109713. DOI: https://doi.org/10.1016/j.foodres.2020.109713

Sadasivam, S., Manickam, A., 1992. Biochemical methods for agricultural sciences. New Delhi, Wiley Eastern Limited, pp.187.

Shah, N.A., Khan, M.R., Naz, K., Khan, M.A., 2014. Antioxidant potential, DNA protection, and HPLC-DAD analysis of neglected medicinal Jurinea dolomiaea roots. BioMed Research International, 2014, 1-10. DOI: https://doi.org/10.1155/2014/726241

Siramon, P., Ohtani, Y., 2007. Antioxidative and antiradical activities of Eucalyptus camaldulensis leaf oils from Thailand. Journal of Wood Science, 53(6), 498-504. DOI: https://doi.org/10.1007/s10086-007-0887-7

Sreeramulu, D., Reddy, C., Raghunath, M., 2009. Antioxidant activity of commonly consumed cereals, millets, pulses and legumes in India. Indian Journal of Biochemistry and Biophysics, 46, 112-115.

Suárez-Martínez, S.E., Ferriz-Martínez, R.A., Campos-Vega, R., Elton-Puente, J.E., de la Torre Carbot, K., García-Gasca, T., 2016. Bean seeds: leading nutraceutical source for human health. CyTA-Journal of Food, 14(1), 131-137. DOI: https://doi.org/10.1080/19476337.2015.1063548

Sultana, B., Anwar, F., Przybylski, R., 2007. Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees. Food Chemistry, 104(3), 1106-1114. DOI: https://doi.org/10.1016/j.foodchem.2007.01.019

Wettasinghe, M., Shahidi, F., 1999. Antioxidant and free radical-scavenging properties of ethanolic extracts of defatted borage (Borago officinalis L.) seeds. Food Chemistry, 67(4), 399-414. DOI: https://doi.org/10.1016/S0308-8146(99)00137-5

Wolfe, K., Wu, X., Liu, R.H., 2003. Antioxidant activity of apple peels. Journal of Agricultural and Food Chemistry, 51(3), 609-614. DOI: https://doi.org/10.1021/jf020782a

You, Y., Duan, X., Wei, X., Su, X., Zhao, M., Sun, J., Jiang, Y., 2007. Identificationof major phenolic compounds of Chinese water chestnut and their antioxidant activity. Molecules, 12(4), 842-852. DOI: https://doi.org/10.3390/12040842

Zhao, G.R., Xiang, Z.J., Ye, T.X., Yuan, Y.J., Guo, Z.X., 2006. Antioxidant activities of Salvia miltiorrhiza and Panax notoginseng. Food Chemistry, 99(4), 767-774. DOI: https://doi.org/10.1016/j.foodchem.2005.09.002

Zhu, Z., Jiang, W., Thompson, H.J., 2012. Edible dry bean consumption (Phaseolus vulgaris L.) modulates cardiovascular risk factors and diet-induced obesity in rats and mice. British Journal of Nutrition, 108(S1), S66-S73. DOI: https://doi.org/10.1017/S0007114512000839

Zou, Y.P., Chang, S.K.C., 2014. Antioxidant and antiproliferative properties of extract and fractions from small red bean (Phaseolus vulgaris L.). Journal of Food and Nutrition, 1(105), 1-11.